The behavior of dihydropyrazine with DNA strand-breakage activity in vivo.
The behavior of dihydropyrazine with DNA strand-breakage activity in vivo.
复制标题
二氢吡嗪在体内具有 DNA 链断裂活性的行为。
DOI：
10.1248/bpb.26.1523
复制
发表时间：
2003
影响因子：
2
通讯作者：
Y. Karube
中科院分区：
文献类型：
--
作者：
Tadatoshi Yamaguchi;Hajime Nomura;K. Matsunaga;S. Ito;J. Takata;Y. Karube
用2，3-二氢-5，6-二甲基吡嗪及其衍生物处理的细胞系的活力的影响，其中揭示了DNA链断裂活性的自由基在体外的产生，从某些形态学变化和凋亡相关蛋白的检测：裂解的PARP和SAPK/JNK。这些结果表明，糖衍生的二氢吡嗪类化合物诱导某些器官细胞的变化，并在体内引起各种内部损伤。本文研究了~（14）C标记的2，3-二氢-5，6-二甲基吡嗪在小鼠体内的生物分布，放射自显影结果有高度的对比。脑、脊髓、唾液腺和胸腺中的放射性较高，心脏、胃和血液中的放射性较低。活性（每个器官的剂量%）支持该结果。
The effects on the viability of cell lines treated with 2,3-dihydro-5,6-dimethylpyrazine and its derivatives, which revealed DNA strand-breakage activity by the generation of radicals in vitro, were recognized from certain morphological changes and the detection of apoptosis-related proteins: cleaved PARP and SAPK/JNK. These results would suggest that sugar-derived dihydropyrazines induce changes in the cells of certain organs and cause various internal injuries in vivo. The biodistribution of 14C-labelled 2,3-dihydro-5,6-dimethylpyrazine was studied in mouse and the autoradiograms showed highly contrasting results. Radioactivity was high in the brain, spinal cord, salivary gland, and thymus and low in the heart, stomach, and blood. The result was supported by the activity (% dose per organ).