Cloned 3T6 cell line from CD-1 mouse fetal molar dental papillae
Cloned 3T6 cell line from CD-1 mouse fetal molar dental papillae
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DOI：
10.3109/03008209809002442
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发表时间：
1998-01-01
影响因子：
2.9
通讯作者：
Butler, WT
中科院分区：
文献类型：
--
作者：
Hanks, CT;Sun, ZL;Butler, WT
文献中只有原代牙髓细胞培养和一种病毒转化小鼠细胞培养被正式报道合成了牙本质基质特有的蛋白，如磷酸蛋白。本研究采用18-19胎日龄CD-1小鼠下颌第一磨牙的牙乳头细胞进行混合培养，并采用3T6培养方案，28传代后进行克隆。该克隆细胞系（MDPC-23）表现出几个独特的特征，其中一些特征是体内成牙细胞的特征。该细胞系的特征包括：(1)所有细胞的上皮样形态具有多个细胞膜突起；(2)所有细胞的碱性磷酸酶活性高；(3)在抗坏血酸和β -甘油磷酸酯中维持时形成多层结节和多层培养物；(4)表达两种成牙本质分化标志物，即牙本质磷蛋白和牙本质唾液蛋白。
Only primary pulpal cell cultures and one virally transformed mouse cell culture have been formally reported in the literature to synthesize proteins such as phosphophoryn which are unique to dentin matrix. In the present study, a mixed culture was derived from dental papilla cells of 18-19 fetal day CD-1 mouse mandibular first molars, maintained on a 3T6 plating regimen, and subsequently cloned after 28 passages. This cloned cell line (MDPC-23) exhibited several unique features, some of which were characteristic of odontoblasts in vivo. The features of this cell line included (1) epithelioid morphology of all cells with multiple cell membrane processes, (2) high alkaline phosphatase activity in all cells, (3) formation of multilayered nodules and multilayered cultures when maintained in ascorbic acid and beta-glycerophosphate, and (4) expression of two markers for odontoblast differentiation, i.e. dentin phosphoprotein and dentin sialoprotein.