Molecular characterization of fungal, community dynamics in the initial stages of composting
Molecular characterization of fungal, community dynamics in the initial stages of composting
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DOI：
10.1016/j.femsec.2004.08.009
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发表时间：
2005-01-01
影响因子：
4.2
通讯作者：
Walker, LP
中科院分区：
文献类型：
--
作者：
Hansgate, AM;Schloss, PD;Walker, LP
堆肥依靠一个复杂的细菌和真菌网络来处理粗糙的有机材料。虽然已知这些生物驱动温度和pH值的动态变化，但对真菌种群在嗜热条件下的时间动态知之甚少。本研究采用F-ARISA（真菌自动rRNA基因间隔分析）和18S rRNA基因克隆和测序来研究这一时期的群落结构变化。对克隆的F-ARISA产物的18S rRNA部分进行测序，发现存在4个不同的真菌属，包括Backusella sp.、Mucoraceae、Geotrichum sp.和酵母Pichia sp.。基于这些ARISA操作分类单元（a - otus）的存在和缺失，我们观察到真菌群落结构在48 - 60 h之间发生了变化。这种变化发生在pH升高之前，与温度升高一致。利用真菌特异性18S rRNA引物构建的克隆文库包含与青霉菌、曲霉、Hamigera、神经孢子菌和酵母菌等真菌属相似的序列。真菌物种丰富度在任何时间点都相对较低，但群落结构是动态的，与细菌群落结构的变化是平行的。(C) 2004年欧洲微生物学会联合会。Elsevier B.V.版权所有。
Composting relies on a complex network of bacteria and fungi to process crude organic material. Although it is known that these organisms drive dynamic changes in temperature and pH, little is known about the temporal dynamics of fungal populations during the rise to thermophilic conditions. This study employed F-ARISA (fungal-automated rRNA intergenic spacer analysis) and 18S rRNA gene cloning and sequencing to examine changes in community structure during this period. Sequencing of the 18S rRNA portion of cloned F-ARISA products revealed the presence of four distinct fungal genera including Backusella sp., Mucoraceae, Geotrichum sp. and the yeast Pichia sp. Based on the presence and absence of these ARISA operational taxonomic units (A-OTUs), we observed a shift in fungal community structure between 48 and 60 h. This change in community structure preceded a rise in pH and coincided with an increase in temperature. Clone libraries constructed using fungi-specific 18S rRNA primers contained sequences similar to several other fungal genera including Penicillium sp., Aspergillus sp., Hamigera sp., Neurospora sp. and the yeast Candida sp. While the fungal species richness was relatively low at any time point, the community structure was dynamic and paralleled changes in bacterial community structure. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.