On the Mechanism of Sodium Extrusion across the Irrigated Gill of Sea Water-Adapted Rainbow Trout (Salmo gairdneri)

On the Mechanism of Sodium Extrusion across the Irrigated Gill of Sea Water-Adapted Rainbow Trout (Salmo gairdneri)
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海水适应虹鳟(Salmo gairdneri)灌溉鳃排钠机制的研究

DOI:
10.1085/jgp.64.2.148
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发表时间:
1974
期刊:
The Journal of General Physiology
影响因子:
--
通讯作者:
M. Sanders
M. Sanders
中科院分区:
--
文献类型:
--
作者:
L. B. Kirschner;L. Greenwald;M. Sanders

文献摘要

被引文献

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在海水(SW)中,通过灌溉鳟鱼鳃的钠流出(J out Na)是快速的,但在淡水(FW)中只有大约25%。这种差异与鳃电位差(TEP)的变化相关。后者在SW中约为+10 mV(血液阳性),而在FW中为-40 mV。通量和电气数据表明,在这条鱼的鳃是可渗透的各种阳离子,包括Na+,K+,Mg 2+,胆碱,和三羟甲基氨基甲烷。它们对阴离子的渗透性较低; P Na:P K:P Cl估计为1:10:0.3,P Cl > P葡萄糖酸盐。TEP被证明是由这些渗透率和SW,FW以及在其他介质中的现存离子梯度确定的扩散电位。在所有进行的实验中,J out Na似乎都是扩散性的。不需要假定交换扩散,是否存在活跃成分的问题仍然悬而未决。
Sodium efflux (J out Na) across the irrigated trout gill was rapid in sea water (SW), but only about 25 % as large in fresh water (FW). The difference correlated with a change in the potential difference across the gill (TEP). The latter was about +10 mV (blood positive) in SW, but –40 mV in FW. Both flux and electrical data indicated that gills in this fish are permeable to a variety of cations including Na+, K+, Mg2+, choline, and Tris. They are less permeable to anions; P Na:P K:P Cl was estimated to be 1:10:0.3, and P Cl > P gluconate. The TEP was shown to be a diffusion potential determined by these permeabilities and the extant ionic gradients in SW, FW as well as in other media. J out Na appeared to be diffusive in all of the experiments undertaken. Exchange diffusion need not be posited, and the question of whether there is an active component remains open.