Characterization of pea aphid resistance in Medicago truncatula
Characterization of pea aphid resistance in Medicago truncatula
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DOI：
10.1104/pp.107.111971
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发表时间：
2008-03-01
期刊：
影响因子：
7.4
通讯作者：
Singh, Karam B.
中科院分区：
文献类型：
--
作者：
Gao, Ling-Ling;Klingler, John P.;Singh, Karam B.
为了深入了解植物-蚜虫相互作用，有必要详细研究植物和昆虫方面的相互作用。豌豆蚜（PA;豌豆蚜（Acyrthosiphon pisum）已被一个国际联合会选为遗传学和基因组学研究的模式种，模式豆科植物蒺藜苜蓿（Medicago truncatula）是该蚜虫的寄主。在这项研究中，我们确定了耐PA的M。truncatula line，'Jester'，具有对密切相关的蚜虫蓝绿蚜虫（BGA; Acyrthosiphon kondoi）的良好表征的抗性。对两种蚜虫的抗性生物学具有相似性，在两种情况下，抗性都发生在韧皮部水平，需要完整的植物，并涉及抗异种性，抗蚜性和植物耐受性的组合。此外，PA抗性共分离在'Jester'与一个单一的显性基因的BGA抗性。这些结果提出了两种抗性可能由相同的机制介导的可能性。这是不支持的基因诱导研究的结果，和BGA诱导的抗性对PA喂养没有影响。此外，含有来自同一抗性供体的BGA抗性基因的不同遗传背景对PA的抗性不同。这些结果表明，不同的机制参与了对这两种蚜虫的抗性。对PA和BGA的抗性遗传背景相同。truncatula使这种植物成为一种有吸引力的模式，用于研究植物和蚜虫的抗和感病植物-蚜虫相互作用的成分。
To achieve a thorough understanding of plant-aphid interactions, it is necessary to investigate in detail both the plant and insect side of the interaction. The pea aphid (PA; Acyrthosiphon pisum) has been selected by an international consortium as the model species for genetics and genomics studies, and the model legume Medicago truncatula is a host of this aphid. In this study, we identified resistance to PA in a M. truncatula line, 'Jester', with well-characterized resistance to a closely related aphid, the bluegreen aphid (BGA; Acyrthosiphon kondoi). The biology of resistance to the two aphid species shared similarity, with resistance in both cases occurring at the level of the phloem, requiring an intact plant and involving a combination of antixenosis, antibiosis, and plant tolerance. In addition, PA resistance cosegregated in 'Jester' with a single dominant gene for BGA resistance. These results raised the possibility that both resistances may be mediated by the same mechanism. This was not supported by the results of gene induction studies, and resistance induced by BGA had no effect on PA feeding. Moreover, different genetic backgrounds containing a BGA resistance gene from the same resistance donor differ in resistance to PA. These results suggest that distinct mechanisms are involved in resistance to these two aphid species. Resistance to PA and BGA in the same genetic background in M. truncatula makes this plant an attractive model for the study of both plant and aphid components of resistant and susceptible plant-aphid interactions.