Co-operative roles for DNA supercoiling and nucleoid-associated proteins in the regulation of bacterial transcription
Co-operative roles for DNA supercoiling and nucleoid-associated proteins in the regulation of bacterial transcription
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DOI：
10.1042/bst20120222
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发表时间：
2013-04-01
影响因子：
3.9
通讯作者：
Dorman, Charles J.
中科院分区：
文献类型：
--
作者：
Dorman, Charles J.
DNA超螺旋和NAPs（nucleotid-associated proteins）参与了许多细菌基因的转录调控。水平获得的SPI（Salmonella pathogenicity island，沙门氏菌致病岛）基因对DNA弛豫反应积极，它们分别被Fis（factor for inversion stimulation，倒置刺激因子）和H-NS（histone like nucleoid-structuring，组蛋白样核结构）NAP激活和抑制，并受到OmpR全局调节蛋白的积极调控。ompR基因是自动调节的，对DNA松弛有积极的反应。Fis和OmpR蛋白与DNA中其靶点的结合对其拓扑状态具有不同的敏感性，而H-NS的结合与DNA的拓扑状态无关。这些数据说明了NAP和DNA拓扑结构对细菌转录控制的贡献的重叠和复杂性。
DNA supercoiling and NAPs (nucleoid-associated proteins) contribute to the regulation of transcription of many bacterial genes. The horizontally acquired SPI (Salmonella pathogenicity island) genes respond positively to DNA relaxation, they are activated and repressed by the Fis (factor for inversion stimulation) and H-NS (histone-like nucleoid-structuring) NAPs respectively, and are positively controlled by the OmpR global regulatory protein. The ompR gene is autoregulated and responds positively to DNA relaxation. Binding of the Fis and OmpR proteins to their targets in DNA is differentially sensitive to its topological state, whereas H-NS binds regardless of the topological state of the DNA. These data illustrate the overlapping and complex nature of NAP and DNA topological contributions to transcription control in bacteria.