A study on the mechanism of agonists in regulating transcriptional level of pIgR in salivary gland epithelial cells
A study on the mechanism of agonists in regulating transcriptional level of pIgR in salivary gland epithelial cells
复制标题
激动剂调节唾液腺上皮细胞pIgR转录水平的机制研究
DOI：
10.3892/etm.2018.6792
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发表时间：
2018-12-01
影响因子：
2.7
通讯作者：
Liu，Zhiming
中科院分区：
文献类型：
--
作者：
Huang，Li;Sun，Chuankong;Liu，Zhiming
本研究旨在探讨激动剂调节唾液腺上皮细胞聚合免疫球蛋白受体(PIgR)转录水平的机制，从而揭示唾液免疫对口腔细菌的防御作用。随机选择2015年4月至2017年4月在武汉大学人民医院就诊的口腔细菌感染患者60例和口腔疾病非细菌感染患者70例。从所有患者唾液腺上皮细胞中提取核糖核酸(RNA)。采用荧光定量聚合酶链式反应(FQ-PCR)和免疫印迹法检测和比较pIgR的转录水平。对60例口腔细菌感染患者的唾液腺上皮细胞进行分离、提取，随机分为观察组和对照组。观察组加入激动剂作用24 h，采用荧光定量聚合酶链式反应(FQ-PCR)和免疫荧光法(IF)检测并比较激动剂作用后pIgR转录水平。用细胞计数试剂盒-8(CCK-8)检测激动剂对细胞的毒性。分离的唾液腺上皮细胞形态符合上皮细胞形态，贴壁生长。细菌感染组pIgR转录水平低于非细菌感染组(p<；0.05)。经激动剂作用后，观察组pIgR转录水平高于对照组(p<；0.05)。激动剂可促进唾液腺上皮细胞pIgR转录水平的升高，pIgR的升高与口腔细菌感染的治愈密切相关。因此，激动剂可以通过调节pIgR的转录来改善口腔免疫功能。
The aim of the present study was to explore the mechanism of agonists in regulating transcriptional level of polymeric immunoglobulin receptor (pIgR) in salivary gland epithelial cells, thus revealing the defense effect of salivary immune on bacteria in the oral cavity. Sixty patients with oral bacterial infection and 70 patients suffering from oral diseases without bacterial infection were selected randomly from patients in Renmin Hospital of Wuhan University from April 2015 to April 2017. Ribonucleic acid (RNA) was extracted from salivary gland epithelial cells of all patients. Fluorescent quantitative polymerase chain reaction (FQ-PCR) and western blotting methods were adopted to detect and compare the transcriptional level of pIgR. The salivary gland epithelial cells of the 60 patients with oral bacterial infection were isolated and extracted, and they were divided into two groups (observation group and control group) randomly. Agonists were added to the observation group for acting for 24 h. FQ-PCR and immunofluorescence (IF) were adopted to detect and compare the transcriptional level of pIgR after acting with agonists. The toxicity of agonists on the cells was detected with Cell Counting kit-8 (CCK-8). The isolated salivary gland epithelial cells conformed to the morphology of epithelial cells, and adhered to the wall for growing. The transcriptional level of pIgR in the bacterial infection group was lower than that in the non-bacterial infection group (p<0.05). The transcriptional level of pIgR in the observation group was higher than that in the control group (p<0.05) after acting with agonists. Agonists can promote the rise of transcriptional level of pIgR in salivary gland epithelial cells, and the increase in pIgR is closely related to the cure of oral bacterial infection. Therefore, agonists can improve the oral immune function by regulating the transcription of pIgR.