Dissipation of the antibiotic sulfamethoxazole in a soil amended with anaerobically digested cattle manure.
Dissipation of the antibiotic sulfamethoxazole in a soil amended with anaerobically digested cattle manure.
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DOI：
10.1016/j.jhazmat.2019.120769
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发表时间：
2019-10
影响因子：
13.6
通讯作者：
J. Rauseo;J. Rauseo;A. Caracciolo;N. Ademollo;Martina Cardoni;M. D. Lenola;William H. Gaze;I. Stanton;P. Grenni;T. Pescatore;T. Pescatore;F. Spataro;L. Patrolecco
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文献类型：
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作者：
J. Rauseo;J. Rauseo;A. Caracciolo;N. Ademollo;Martina Cardoni;M. D. Lenola;William H. Gaze;I. Stanton;P. Grenni;T. Pescatore;T. Pescatore;F. Spataro;L. Patrolecco
在农业用地上施用厌氧消化的牛粪，既可以提高牛粪的质量，又可以回收利用农场废物，这是符合循环经济的一种日益普遍的做法。然而，关于通过这种具体做法传播抗生素耐药性的潜在风险的知识相当匮乏。抗生素磺胺甲恶唑（SMX）是兽药中最常用的处方之一。在本研究中，研究了SMX在畜牧场内沼气厂生产的牛粪厌氧消化土壤中的耗散及其对天然微生物可能产生的影响。微观实验采用SMX（20 mg/kg土壤）处理改良土壤。在试验期间（61 d），对土壤样品进行SMX、n4 -乙酰磺胺甲恶唑、微生物丰度、活性和结构分析。此外，还确定了inti1基因的流行率。总体结果表明，虽然最初对微生物丰度有负面影响，但SMX在大约7天内减少了一半。在消化液和改良土壤中均发现了inti1基因，这表明厌氧消化牛粪作为肥料可能是农业生态系统中抗生素耐药细菌（ARBs）和基因（ARGs）的来源。
The application of anaerobically digested cattle manure on agricultural land for both improving its quality and recycling a farm waste is an increasingly frequent practice in line with the circular economy. However, knowledge on the potential risk of spreading antibiotic resistance through this specific practice is quite scarce. The antibiotic sulfamethoxazole (SMX) is one of the most heavily prescribed in veterinary medicine. In this study, SMX dissipation and the possible effects on natural microorganisms were investigated in a soil amended with an anaerobically digested cattle manure produced from a biogas plant inside a livestock farm. Microcosm experiments were performed using amended soil treated with SMX (20 mg/kg soil). During the experimental time (61 days), soil samples were analysed for SMX and N4-acetylsulfamethoxazole, microbial abundance, activity and structure. Furthermore, the prevalence of theintI1gene was also determined. The overall results showed that, although there was an initial negative effect on microbial abundance, SMX halved in about 7 days in the digestate-amended soil. TheintI1gene found in both the digestate and amended soil suggested that the use of anaerobically digested cattle manure as fertilizer can be a source of antibiotic resistant bacteria (ARBs) and genes (ARGs) in agroecosystems.