Glycol methacrylate embedding for improved morphological, morphometrical, and immunohistochemical investigations under light microscopy: testes as a model.

Glycol methacrylate embedding for improved morphological, morphometrical, and immunohistochemical investigations under light microscopy: testes as a model.
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甲基丙烯酸乙二醇酯包埋可改善光学显微镜下的形态学、形态测量和免疫组织化学研究:以睾丸为模型。

DOI:
10.1007/978-1-60761-950-5_1
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发表时间:
2011
影响因子:
--
通讯作者:
F. Almeida
F. Almeida
中科院分区:
--
文献类型:
--
作者:
H. Chiarini;G. G. Parreira;F. Almeida

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甲基丙烯酸乙二醇酯(GMA)是一种水和乙醇混溶的塑料树脂,是一种方便用于光学显微镜包埋的介质,比石蜡包埋具有许多优点。GMA改善了组织学、形态计量学和免疫组化评价,主要是由于对细胞学细节的准确评估。本章重点介绍我们在GMA处理方面的经验,并详细描述了我们用于睾丸评价的固定、包埋和染色方法。
Glycol methacrylate (GMA), a water and ethanol miscible plastic resin, is a medium handy to use for light microscopy embedding that has a number of advantages than paraffin embedding. The GMA improves the histological, morphometrical, and immunohistochemical evaluations, mainly due to the accurate assessment of cytological details. This chapter focuses on our experience in the GMA processing and describes in detail the fixation, embedding, and staining methods that we have been using for testes evaluations.
大鼠精原细胞的非随机分布:生精小管中生态位的证据。
DOI: 10.1530/rep.0.1260669
发表时间: 2003
期刊: Reproduction (Cambridge, England)
影响因子: --
作者:
Chiarini-Garcia,H;Raymer,AM;Russell,LD
通讯作者: Russell,LD