Mitogen-Activated Protein Kinase Activity Is Not Essential for the First Step of Nuclear Reprogramming in Bovine Somatic Cell Nuclear Transfer.
Mitogen-Activated Protein Kinase Activity Is Not Essential for the First Step of Nuclear Reprogramming in Bovine Somatic Cell Nuclear Transfer.
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DOI：
10.1089/cell.2016.0044
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发表时间：
2017-04
影响因子：
1.6
通讯作者：
T. Tani;Y. Kato
中科院分区：
文献类型：
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作者：
T. Tani;Y. Kato
在哺乳动物克隆过程中，为了对体细胞核进行重编程，第二次减数分裂中期卵母细胞被广泛用作受体细胞质。成熟促进因子(MPF)和丝裂原活化蛋白激酶(MAPK)的高活性被认为加速了通过体细胞核移植进入卵质的体细胞核的重塑和/或重编程。我们先前已经证明，核重新编程的第一步不是由MPF和MAPK直接调节的，因为在牛克隆中，MPF活性降低而MAPK活性保持的激活卵母细胞可以在获得M期体细胞核后发育到囊胚期。在这项研究中，我们的目的是测试MAPK活性是否是牛体细胞克隆中核重编程和/或染色质重塑(组蛋白H3在Ser3处的磷酸化，组蛋白H3在Lys 9处的三甲基化，以及组蛋白H3在Lys14处的乙酰化)的第一步所必需的。我们发现这是没有必要的，强积金活动也是没有必要的。
For reprogramming a somatic nucleus during mammalian cloning, metaphase of the second meiotic division (MII) oocytes has been widely used as recipient cytoplasm. High activity of maturation-promoting factor (MPF) and mitogen-activated protein kinase (MAPK) is believed to accelerate the remodeling and/or reprogramming of a somatic nucleus introduced into the ooplasm by somatic cell nuclear transfer. We demonstrated previously that the first step in nuclear reprogramming is not directly regulated by MPF and MAPK because activated oocytes in which MPF activity is diminished and MAPK activity is maintained can develop to the blastocyst stage after receiving an M phase somatic nucleus in bovine cloning. In this study, our aim was to test whether MAPK activity is necessary for the first step in nuclear reprogramming and/or chromatin remodeling (phosphorylation of histone H3 at Ser3, trimethylation of histone H3 at Lys 9, and acetylation of histone H3 at Lys14) in bovine somatic cloning. We found that it was not necessary, and neither was MPF activity.