Bilateral cervical contusion spinal cord injury in rats
Bilateral cervical contusion spinal cord injury in rats
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DOI：
10.1016/j.expneurol.2009.06.012
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发表时间：
2009-11-01
影响因子：
5.3
通讯作者：
Steward, Oswald
中科院分区：
文献类型：
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作者：
Anderson, Kim D.;Sharp, Kelli G.;Steward, Oswald
人们越来越有动力开发啮齿类动物颈椎损伤的临床相关实验模型和评估前肢功能缺陷的技术。在这里，我们描述了大鼠双侧颈挫伤模型。雌性 Sprague-Dawley 大鼠在 C5、C6 或 C7/8 处受到轻度或中度颈挫伤（使用 Infinite Horizons 装置）。使用握力计（GSM）评估前肢运动功能；通过冯弗雷毛发测试评估感觉功能；通过生物素化葡聚糖胺 (BDA) 束追踪评估皮质脊髓束 (CST) 的完整性。轻度挫伤主要造成背柱（DC）和灰质（GM）损伤，而中度挫伤会对侧向和腹侧组织造成额外损伤。受伤后，前肢和后肢功能立即严重受损，但所有大鼠都恢复了使用后肢运动的能力。受伤后抓握能力立即消失，但部分恢复，具体取决于脊柱水平和受伤的严重程度。大鼠的前肢和后肢均出现感觉丧失，但部分恢复，并且未表现出异常性疼痛。束追踪显示，除了一只动物外，DC 中 CST 轴突的主要部分在所有动物中都完全中断，而背外侧 CST (dICST) 部分幸免，并且 dICST 轴突产生在损伤后的 GM 尾部形成树状的轴突。我们的数据表明，大鼠可以在 C5 或以下的严重双侧颈挫伤中幸存，并且即使背柱 CST 轴突的主要组成部分完全中断，前爪抓握功能也会在轻度损伤后恢复。 (C) 2009 Elsevier Inc. 保留所有权利。
There is increasing motivation to develop clinically relevant experimental models for cervical SCI in rodents and techniques to assess deficits in forelimb function. Here we describe a bilateral cervical contusion model in rats. Female Sprague-Dawley rats received mild or moderate cervical contusion injuries (using the Infinite Horizons device) at C5, C6, or C7/8. Forelimb motor function was assessed using a grip strength meter (GSM); sensory function was assessed by the von Frey hair test; the integrity of the corticospinal tract (CST) was assessed by biotinylated dextran amine (BDA) tract tracing. Mild contusions caused primarily dorsal column (DC) and gray matter (GM) damage while moderate contusions produced additional damage to lateral and ventral tissue. Forelimb and hindlimb function was severely impaired immediately post-injury, but all rats regained the ability to use their hindlimbs for locomotion. Gripping ability was abolished immediately after injury but recovered partially, depending upon the spinal level and severity of the injury. Rats exhibited a loss of sensation in both fore- and hindlimbs that partially recovered, and did not exhibit allodynia. Tract tracing revealed that the main contingent of CST axons in the DC was completely interrupted in all but one animal whereas the dorsolateral CST (dICST) was partially spared, and dICST axons gave rise to axons that arborized in the GM caudal to the injury. Our data demonstrate that rats can survive significant bilateral cervical contusion injuries at or below C5 and that forepaw gripping function recovers after mild injuries even when the main component of CST axons in the dorsal Column is completely interrupted. (C) 2009 Elsevier Inc. All rights reserved.