MHC and sarcoplasmic reticulum protein isoforms in functionally overloaded cat plantaris muscle fibers.
MHC and sarcoplasmic reticulum protein isoforms in functionally overloaded cat plantaris muscle fibers.
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功能超载的猫跖肌纤维中的 MHC 和肌浆网蛋白亚型。
DOI：
10.1152/jappl.1996.80.4.1296
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Edgerton,VR
中科院分区：
文献类型：
--
作者：
Talmadge,RJ;Roy,RR;Chalmers,GR;Edgerton,VR
为了确定功能过载（FO）后肌球蛋白重链（MHC）亚型表达的适应是否伴随着单根肌纤维中负责松弛的蛋白质亚型[肌浆网（SR）、Ca（2+）-腺苷三磷酸酶（SERCA）和受磷蛋白（PHL）]的相应适应，在FO后3个月测定猫跖肌纤维的MHC和SERCA的同种型以及PHL的存在或不存在。在控制跖的相对MHC亚型组成为23%的I型，21%的IIa型，和56%的IIb型。FO导致向较慢亚型转变（33% I型，44% IIa型和23% IIb型）。在深区域的跖肌的比例I型MHC和混合型MHC纤维（包含I型和II型MHC）分别为40和200%以上的FO猫，分别。FO导致仅含有慢SERCA同种型（SERCA 2）的纤维的比例增加47%，并且含有PHL的纤维的比例增加41%。对照组和跖足中含I型MHC、SERCA 2和PHL的纤维比例呈线性相关。这些数据表明，适应MHC亚型表达伴随着相称的适应肌浆网蛋白亚型在单肌纤维后FO。
To determine whether the adaptations in myosin heavy chain (MHC) isoform expression after functional overload (FO) are accompanied by commensurate adaptations in protein isoforms responsible for relaxation [sarco(endo)plasmic reticulum (SR) Ca(2+)-adenosinetriphosphatase (SERCA) and phospholamban (PHL)] in single muscle fibers, the isoforms of MHC and SERCA and the presence or absence of PHL were determined for cat plantaris fibers 3 mo after FO. In control plantaris the relative MHC isoform composition was 23% type I, 21% type IIa, and 56% type IIb. FO resulted in a shift toward slower isoforms (33% type I, 44% type IIa, and 23% type IIb). In the deep region of the plantaris the proportions of type I MHC and hybrid MHC fibers (containing type I and II MHCs) were 40 and 200% greater in FO cats, respectively. FO resulted in a 47% increase in the proportion of fibers containing only the slow SERCA isoform (SERCA2) and a 41% increase in the proportion of fibers containing PHL. The proportions of fibers containing type I MHC, SERCA2, and PHL in control and FO plantaris were linearly correlated. These data show that adaptations in MHC isoform expression are accompanied by commensurate adaptations in sarcoplasmic reticulum protein isoforms in single muscle fibers after FO.