Real time in situ confocal imaging of calcium wave in the perfused whole heart of the rat
Real time in situ confocal imaging of calcium wave in the perfused whole heart of the rat
复制标题
DOI：
10.1016/s0898-6568(97)00136-8
复制
发表时间：
1998-05-01
影响因子：
4.8
通讯作者：
Takamatsu, T
中科院分区：
文献类型：
--
作者：
Hama, T;Takahashi, A;Takamatsu, T
为了了解具有窦房结自动性的全心钙离子处理，我们开发了一种大鼠全心灌注细胞内钙离子浓度的原位成像系统。该系统由一个安装有多针孔型实时共聚焦激光扫描装置的固定台立式显微镜和一个用于观察的水浸物镜组成。这种原位成像系统使观察和分析细胞内钙动态的快速变化的图像成为可能，在整个大鼠心脏负荷fluo-3。扫描以每秒30帧的视频速率进行，共聚焦效应包括X和Y植物。钙波经常被外部电刺激脉冲或自发窦性心律引起的钙瞬态打断。我们的研究结果表明，如果心肌细胞有有序的端对端细胞间电通路，那么微小区域的异常钙波不会干扰整个心脏的兴奋-收缩耦合。(C) 1998爱思唯尔科学有限公司
To understand the calcium handling in whole heart having automaticity of the sinus node, we have developed a system of in situ imaging the intracellular calcium ion concentration in the perfused whole heart of the rat. The system consists of a stage-fixed upright microscope equipped with a real-time confocal laser scanning device of a multipinhole type with a water-immersion objective lens for observation. This in situ imaging system rendered observations and analyses of the rapidly changing images of intracellular calcium dynamics possible in the whole rat heart loaded with fluo-3. The scanning was conducted at a video rate of 30 frames per second, and the confocal effects included both X and Y plants. Calcium waves were frequently interrupted by calcium transients from either external electro-stimulation pulses or spontaneous sinus rhythm. Our findings suggest that abnormal calcium waves in minute areas cannot disturb the excitation-contraction coupling in the whole heart if the myocardial cells have orderly end-on-end intercellular electric paths. (C) 1998 Elsevier Science Inc.