INCORPORATION OF [C-14] GLUCOSE INTO CRYSTALLINE CELLULOSE IN ABERRANT FIBERS OF A COTTON MUTANT
INCORPORATION OF [C-14] GLUCOSE INTO CRYSTALLINE CELLULOSE IN ABERRANT FIBERS OF A COTTON MUTANT
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DOI：
10.2135/cropsci1993.0011183x003300050032x
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发表时间：
1993-09-01
期刊：
影响因子：
2.3
通讯作者：
JIVIDEN, GM
中科院分区：
文献类型：
--
作者：
KOHEL, RJ;BENEDICT, CR;JIVIDEN, GM
Ligon lintless-1是棉花（Gossypium hirsutum L.）的显性单遗传突变体，含有明显短的棉纤维和广泛增厚的次生壁。研究了[C-14]葡萄糖在棉花初生和次生壁结晶纤维素中的掺入，以确定结晶纤维素微纤维的形成程度和微纤维沉积方式与突变棉异常生长发育模式的关系。结果表明，突变体纤维初生壁中结晶纤维素的形成速率与纤维伸长率和初生壁形成速率的降低有关。与野生型纤维相比，突变型纤维在次生壁形成过程中每毫米纤维长度形成的结晶纤维素的速率增加了五倍。Ligon lintless-1基因突变影响棉纤维的生长发育，并改变棉纤维初生和次生壁中结晶纤维素微原纤维的形成速率。次生壁中结晶纤维素微原纤维的增加很可能是由于单个纤维素合酶复合物的合成活性增加或突变体中每单位纤维长度的合成复合物位点数量增加所致。
Ligon lintless-1 is a dominant simply inherited mutant of cotton (Gossypium hirsutum L.) containing markedly short cotton fibers with extensively thickened secondary walls. The incorporation of [C-14]glucose into crystalline cellulose in the primary and secondary walls was studied to determine the relation between the extent of crystalline cellulose microfibril formation and the pattern of microfibril deposition to the aberrant growth and developmental pattern in the mutant cotton. The results show that the rate of crystalline cellulose formation in the primary walls of the mutant fibers correlates with the reduced rate of fiber elongation and primary wall formation. There is a five-fold increase in the rate of crystalline cellulose formed per millimeter of fiber length during secondary wall formation in the mutant fibers compared to the rate in the wild-type fibers. The Ligon lintless-1 gene mutation affects the growth and development of the cotton fibers with accompanying changes in the rate of formation of crystalline cellulose microfibrils in the primary and secondary walls. This increase in crystalline cellulose microfibrils in secondary walls is most likely due either to an increase in synthetic activity of the individual cellulose synthase complexes or to an increase in number of synthetic complex sites per unit of fiber length in the mutant.