The Path from β-Carotene to Carlactone, a Strigolactone-Like Plant Hormone
The Path from β-Carotene to Carlactone, a Strigolactone-Like Plant Hormone
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DOI：
10.1126/science.1218094
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发表时间：
2012-03-16
期刊：
影响因子：
56.9
通讯作者：
Al-Babili, Salim
中科院分区：
文献类型：
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作者：
Alder, Adrian;Jamil, Muhammad;Al-Babili, Salim
独脚金内酯是具有多种信号活性的植物激素，具有由两个通过烯醇醚桥连接的内酯组成的共同结构。独脚金内酯通过涉及类胡萝卜素裂解双加氧酶 7 和 8（CCD7 和 CCD8）以及铁结合蛋白 D27 的途径衍生自类胡萝卜素。我们证明D27是一种β-胡萝卜素异构酶，可将全反式-β-胡萝卜素转化为9-顺式-β-胡萝卜素，并被CCD7裂解成9-顺式醛。 CCD8将三个氧原子结合成9-cis-beta-apo-10'-胡萝卜醛，并进行分子重排，将类胡萝卜素与独脚金内酯连接起来，生成卡拉内酯，这是一种具有类似独脚金内酯生物活性的化合物。了解卡拉内酯的结构对于理解独脚金内酯的生物学至关重要，并且可能在防治寄生杂草方面具有应用。
Strigolactones, phytohormones with diverse signaling activities, have a common structure consisting of two lactones connected by an enol-ether bridge. Strigolactones derive from carotenoids via a pathway involving the carotenoid cleavage dioxygenases 7 and 8 (CCD7 and CCD8) and the iron-binding protein D27. We show that D27 is a beta-carotene isomerase that converts all-trans-beta-carotene into 9-cis-beta-carotene, which is cleaved by CCD7 into a 9-cis-configured aldehyde. CCD8 incorporates three oxygens into 9-cis-beta-apo-10'-carotenal and performs molecular rearrangement, linking carotenoids with strigolactones and producing carlactone, a compound with strigolactone-like biological activities. Knowledge of the structure of carlactone will be crucial for understanding the biology of strigolactones and may have applications in combating parasitic weeds.