Nitric oxide synthase expression in bone cells
Nitric oxide synthase expression in bone cells
复制标题
DOI：
10.1016/s8756-3282(98)00070-2
复制
发表时间：
1998-07-01
期刊：
影响因子：
4.1
通讯作者：
Chow, JWM
中科院分区：
文献类型：
--
作者：
Fox, SW;Chow, JWM
我们使用免疫组织化学定位了大鼠和人类骨细胞中三种主要的一氧化氮合酶（eNOS、bNOS和iNOS）的表达。在正常成人骨组织中，eNOS主要表达于成骨细胞系细胞，eNOS主要表达于扁平骨衬里细胞和骨细胞，而立方骨细胞eNOS表达阴性。在任何骨细胞中均未检测到bNOS的表达。在正常成年大鼠和人骨的成骨细胞系中未检测到iNOS的表达，但在实验性结肠炎的成年大鼠的立方成骨细胞中观察到iNOS的表达，其中骨形成的抑制可能是细胞因子介导的。正常大鼠破骨细胞eNOS和iNOS均有表达，但呈斑片状。至于成骨细胞系的细胞，破骨细胞是阴性的bNOS，因此，我们的研究结果支持证据，从体外研究和动物实验，一氧化氮可能在骨的生理学中发挥重要作用。(Bone 23：1-6; 1998）（C）1998由Elsevier Science Inc保留所有权利。
We have localized the expression of the three main nitric oxide synthases (eNOS, bNOS, and iNOS) in bone cells of rats and humans using immunohistochemistry. The predominant isoform expressed in normal adult bone was the constitutive isoform, eNOS, mainly in cells of osteoblastic lineage, In adult bone, the osteoblast lineage cells exhibiting eNOS expression were flat bone lining cells and osteocytes, but cuboidal osteoblasts were consistently negative. Expression for bNOS was not detected in any bone cells. iNOS expression was not detected in any cells of osteoblastic lineage in normal adult rat or human bone, but was observed in cuboidal osteoblasts of adult rats with experimental colitis, in which the suppression in bone formation may be cytokine mediated. Osteoclasts in normal rat tissue showed expression for both eNOS and iNOS, but these were patchy. As for cells of the osteoblast lineage, osteoclasts were negative for bNOS, Thus, our findings support evidence, from in vitro studies and from animal experiments, that nitric oxide may play an important role in the physiology of bone. (Bone 23:1-6; 1998) (C) 1998 by Elsevier Science Inc All rights reserved.