Modulation of antigen‐specific T‐cell activation in vitro by taurine chloramine
Modulation of antigen‐specific T‐cell activation in vitro by taurine chloramine
复制标题
牛磺酸氯胺体外调节抗原特异性 T 细胞活化
作者：
J. Marcinkiewicz;A. Grabowska;B. Chain
牛磺酸氯胺（TauCl）是在炎症期间通过次氯酸（HOCl）与牛磺酸（中性粒细胞中最丰富的游离氨基酸）反应产生的。我们以前报道过TauCl抑制巨噬细胞炎症介质的产生，如一氧化氮、前列腺素E2（PGE 2）、肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）。在本研究中，研究了TauCl在调节T细胞活化中的活性。发现在活化之前用TauCl（0.1 - 0.3 mm）处理T细胞抑制响应于有丝分裂原和抗原刺激的白细胞介素-2（IL-2）释放。 类似地，发现在低细胞数量下预处理A-20抗原呈递细胞（APC）抑制其加工卵清蛋白（OVA）并将其呈递给特异性T细胞杂交瘤的能力。相比之下，在存在OVA的情况下用TauCl预处理较高数量的A-20细胞增强了随后的OVA呈递。最后，用TauCl改性的OVA比天然VA更有效地加工和呈现。因此，TauCl能够在整个抗原呈递途径的几个独立点调节特异性适应性免疫应答的诱导。
Taurine chloramine (TauCl) is produced during inflammation by reaction of hypochlorous acid (HOCl) with taurine, the most abundant free amino acid in neutrophils. We previously reported that TauCl inhibits the generation of macrophage inflammatory mediators such as nitric oxide, prostaglandin E2 (PGE2),tumour necrosis factor‐α (TNF‐α) and interleukin‐6 (IL‐6). In this study, the activity of TauCl in modulating T‐cell activation was investigated. Treatment of T cells with TauCl (0·1–0·3 mm), prior to activation, was found to inhibit interleukin‐2 (IL‐2) release in response to both mitogen and antigen stimulation. Similarly, pretreatment of A‐20 antigen presenting cells (APCs), at low cell numbers, was found to inhibit their ability to process and present ovalbumin (OVA) to a specific T‐cell hybridoma. In contrast, pretreatment of higher numbers of A‐20 cells with TauCl in the presence of OVA enhanced subsequent presentation of OVA. Finally, OVA modified with TauCl was processed and presented more efficiently than native VA. Thus, TauCl is able to modulate induction of a specific adaptive immune response at several independent points of the overall antigen‐presenting pathway.
牛磺酸氯胺通过对诱导型环氧合酶表达的转录后作用，抑制激活的 RAW 264.7 细胞中前列腺素 E2 的产生。
影响因子：
4.4
作者：
Quinn,MR;Park,E;Schuller-Levis,G
通讯作者：
Schuller-Levis,G
影响因子：
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作者：
Thomas,EL;Grisham,MB;Jefferson,MM
通讯作者：
Jefferson,MM