PLASTID GENES ENCODING THE TRANSCRIPTION TRANSLATION APPARATUS ARE DIFFERENTIALLY TRANSCRIBED EARLY IN BARLEY (HORDEUM-VULGARE) CHLOROPLAST DEVELOPMENT - EVIDENCE FOR SELECTIVE STABILIZATION OF PSBA MESSENGER-RNA
PLASTID GENES ENCODING THE TRANSCRIPTION TRANSLATION APPARATUS ARE DIFFERENTIALLY TRANSCRIBED EARLY IN BARLEY (HORDEUM-VULGARE) CHLOROPLAST DEVELOPMENT - EVIDENCE FOR SELECTIVE STABILIZATION OF PSBA MESSENGER-RNA
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DOI：
10.1104/pp.101.3.781
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发表时间：
1993-03-01
期刊：
影响因子：
7.4
通讯作者：
MULLET, JE
中科院分区：
文献类型：
--
作者：
BAUMGARTNER, BJ;RAPP, JC;MULLET, JE
叶绿体基因组编码与转录、翻译和光合作用有关的rRNA、tRNA和蛋白质。代表这些功能的15个质体基因的表达在大麦（大麦）叶绿体发育过程中进行了定量。所有质体基因的转录在叶绿体发育的初始阶段增加，然后在叶绿体成熟下降。rpoB-rpoC 1-rpoC 2编码质体RNA聚合酶的亚基，rps 16编码核糖体蛋白，在叶绿体发育的早期，在编码光合机构亚基的基因（rbcL，atpB，psaA，petB）之前，rpoB-rpoC 1-rpoC 2对应的RNA达到最大丰度。rpoB以及16 S rRNA，trnfM-trnG，和trnK的转录是高的叶绿体发育早期和下降10倍，相对于rbcL的转录在叶绿体成熟。RNA杂交psbA和psbD，基因编码的反应中心蛋白的光系统II，差异保持在成熟的叶绿体照明大麦。psbD mRNA相对于rbcL mRNA的差异积累是由于光刺激的psbD转录。与此相反，增强水平的psbA mRNA在成熟的叶绿体主要是由于选择性稳定的psbA mRNA。这些数据记录了大麦叶绿体发育过程中质体基因转录和mRNA稳定性的动态调节。
Chloroplast genomes encode rRNAs, tRNAs, and proteins involved in transcription, translation, and photosynthesis. The expression of 15 plastid genes representing each of these functions was quantitated during chloroplast development in barley (Hordeum vulgare). The transcription of all plastid genes increased during the initial phase of chloroplast development and then declined during chloroplast maturation. RNAs corresponding to rpoB-rpoC1-rpoC2, which encode subunits of a plastid RNA polymerase, and rps16, which encodes a ribosomal protein, reached maximal abundance early in chloroplast development prior to genes encoding subunits of the photosynthetic apparatus (rbcL, atpB, psaA, petB). Transcription of rpoB as well as 16S rRNA, trnfM-trnG, and trnK was high early in chloroplast development and declined 10-fold relative to rbcL transcription during chloroplast maturation. RNA hybridizing to psbA and psbD, genes encoding reaction center proteins of photosystem II, was differentially maintained in mature chloroplasts of illuminated barley. Differential accumulation of psbD mRNA relative to rbcL mRNA was due to light-stimulated transcription of psbD. In contrast, enhanced levels of psbA mRNA in mature chloroplasts were due primarily to selective stabilization of the psbA mRNA. These data document dynamic modulation of plastid gene transcription and mRNA stability during barley chloroplast development.