The Caenorhabditis elegans EGL-26 protein mediates vulval cell morphogenesis
The Caenorhabditis elegans EGL-26 protein mediates vulval cell morphogenesis
复制标题
DOI：
10.1006/dbio.2001.0514
复制
发表时间：
2002-01-15
影响因子：
2.7
通讯作者：
Han, M
中科院分区：
文献类型：
--
作者：
Hanna-Rose, W;Han, M
在筛选外阴形态发生缺陷的秀丽隐杆线虫突变体时，我们分离出子宫和外阴不能建立功能连接的多个突变体，导致产卵缺陷表型。其中两个连接性腺缺陷（Cog）突变体携带egl-26基因的等位基因。我们证明了egl-26突变动物的外阴谱系是正常的，但一个外阴细胞vu1F采用了异常的形态。这导致在外阴顶端形成异常厚的外阴组织层，并造成从子宫到外阴出口的物理阻塞。克隆了Egl-26，并预测其编码一种新的蛋白。嵌合分析表明，egl-26活性在vu1F的原始外阴谱系中是必需的。EGL-26与GFP的功能性翻译融合表达仅在与vu1F相邻的vulE中观察到。EGL-26定位于vu1E细胞的顶端边缘。因此，在EGL-26介导的相互作用中，vu1E可能会指导相邻细胞vu1F的形态变化。(C) 2001 Elsevier Science。
In screens for Caenorhabditis elegans mutants defective in vulval morphogenesis, we isolated multiple mutants in which the uterus and the vulva fail to make a functional connection, resulting in an egg-laying defective phenotype. Two of these connection of gonad defective (Cog) mutants carry alleles of the egl-26 gene. We demonstrate that vulval lineages in egl-26 mutant animals are normal, but one vulval cell, vu1F, adopts an abnormal morphology. This results in formation of an abnormally thick layer of vulval tissue at the apex of the vulva and a physical blockage of the exit to the vulva from the uterus. egl-26 was cloned and is predicted to encode a novel protein. Mosaic analysis indicates that egl-26 activity is required in the primary vulval lineage for vu1F morphogenesis. Expression of a functional translational fusion of EGL-26 to GFP was observed within the primary vulval lineage only in vulE, which neighbors vu1F. EGL-26 is localized at the apical edge of the vu1E cell. It is thus possible that vu1E acts to instruct morphological changes in the neighboring cell, vu1F, in an interaction mediated by EGL-26. (C) 2001 Elsevier Science.