An X-encoded alloantigenicity between BALB/c and C57BL/6 strains of mice.
An X-encoded alloantigenicity between BALB/c and C57BL/6 strains of mice.
复制标题
BALB/c 和 C57BL/6 品系小鼠之间的 X 编码同种异体抗原性。
DOI：
10.1007/s00251-003-0554-0
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发表时间：
2003
期刊：
影响因子：
3.2
通讯作者：
King,ThomasR
中科院分区：
文献类型：
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作者：
Alner,Kadie-Ann;Loman,Jeannette;Hall,EmilyH;Mutcherson2nd,RayeJ;King,ThomasR
为了检测小鼠 X 染色体上可能编码的组织相容性的微小障碍，我们将相应组的 (C57BL/6×BALB/c)F1、(C57BL/6×DBA/2)F1 和 (BALB/c×DBA/2)F1 小鼠移植了来自各自父本近交系的尾部皮肤。我们的组织学分析表明，与 C57BL/6 小鼠品系相比，BALB/c 品系会产生 X 连锁抗原丢失。相反，我们在菌株 C57BL/6 和 DBA/2 或 DBA/2 和 BALB/c 之间没有检测到 X 连锁的组织学差异。为了将这种 X 连锁障碍定位到组织相容性，我们制作了一组 25 只 [(BALB/c×C57BL/6)F1×C57BL/6]N2 雄性，将其与 C57BL/6 皮肤移植，以确定哪些携带移植排斥所需的 BALB/c 衍生成分。 DNA标记分析显示移植排斥者中有一个重叠的BALB/c衍生的X染色体片段区域，表明这种抗原丢失单倍型（H-hixc，X染色体上的组织不相容性，c单倍型）可能被限制在DXMit55到Xq端粒间隔（仅排除X的着丝粒尖端）内。 H-hixcto C57BL/6 的进一步回交产生的排斥小鼠比 N4 代预期的要少，这表明第二个未连锁的基因座也参与了这种 X 连锁同种异体抗原性。 (BALB/c×C57BL/6)F1 雄性对雄性 (C57BL/6×BALB)F1 和雌性 (B6.C-H2d×C57BL/6)F1 皮肤的强烈排斥，以及对 N2 和 N4 移植受体雄性中 17 号染色体标记的评估表明，第二个基因座是 H2，并且 H-hixb 编码的同种抗原需要两个 H2bandH2d 编码的递呈分子可实现有效的移植物排斥。
To detect minor barriers to histocompatibility that might be encoded on the X chromosome in mice, we grafted reciprocal sets of (C57BL/6×BALB/c)F1, (C57BL/6×DBA/2)F1, and (BALB/c×DBA/2)F1 mice with tail skin from the respective paternal inbred strain. Our histogenic analysis suggests that, compared with the C57BL/6 mouse strain, the BALB/c strain generates X-linked antigen loss. In contrast, we detected no X-linked histogenic differences between strains C57BL/6 and DBA/2, or DBA/2 and BALB/c. To localize this X-linked barrier to histocompatibility, we produced a panel of 25 [(BALB/c×C57BL/6)F1×C57BL/6]N2 males that were grafted with C57BL/6 skin to determine which carried the BALB/c-derived component(s) necessary for graft rejection. DNA marker analysis showed one region of overlapping BALB/c-derived X-chromosomal segments among the graft rejecters, suggesting that this antigen-loss haplotype (H-hixc, for histoincompatibility on the X chromosome, c haplotype) may be restricted within theDXMit55to the Xq telomere interval (which excludes only the centromeric tip of the X). Further backcrossing ofH-hixcto C57BL/6 resulted in fewer rejecter mice than expected by the N4 generation, suggesting that a second, unlinked locus is also involved in this X-linked alloantigenicity. The vigorous rejection of male (C57BL/6×BALB)F1 and female (B6.C-H2d×C57BL/6)F1 skin by (BALB/c×C57BL/6)F1 males, as well as the assessment of markers on Chromosome 17 among N2 and N4 graft-recipient males, suggests that this second locus isH2, and thatH-hixb-encoded alloantigens require bothH2bandH2d-encoded presentation molecules for efficient graft rejection.