Continued expression of neonatal myosin heavy chain in adult dystrophic skeletal muscle.
Continued expression of neonatal myosin heavy chain in adult dystrophic skeletal muscle.
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新生儿肌球蛋白重链在成人营养不良的骨骼肌中持续表达。
DOI：
10.1126/science.3969567
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Bandman,E
中科院分区：
文献类型：
--
作者：
Bandman,E
用新生肌球蛋白特异性单克隆抗体检测了正常和营养不良鸡肌肉中肌球蛋白重链亚型的表达。成人营养不良的肌肉继续含有新生肌球蛋白后，很长一段时间，它从成人正常肌肉消失。一种新的技术，包括蛋白质印迹和肽图谱表明，免疫反应性肌球蛋白在成人营养不良的肌肉是相同的，在新生儿正常肌肉。免疫细胞化学显示，所有的纤维在营养不良的肌肉未能抑制新生肌球蛋白重链。这些研究表明，肌营养不良症抑制肌球蛋白基因转换，通常发生在肌肉成熟。
The expression of myosin heavy chain isoforms was examined in normal and dystrophic chicken muscle with a monoclonal antibody specific for neonatal myosin. Adult dystrophic muscle continued to contain neonatal myosin long after it disappeared from adult normal muscle. A new technique involving western blotting and peptide mapping demonstrated that the immunoreactive myosin in adult dystrophic muscle was identical to that found in neonatal normal muscle. Immunocytochemistry revealed that all fibers in the dystrophic muscle failed to repress neonatal myosin heavy chain. These studies suggest that muscular dystrophy inhibits the myosin gene switching that normally occurs during muscle maturation.