The Inhibitory Effect of Ergosterol, a Bioactive Constituent of a Traditional Japanese Herbal Medicine Saireito on the Activity of Mucosal-Type Mast Cells
The Inhibitory Effect of Ergosterol, a Bioactive Constituent of a Traditional Japanese Herbal Medicine Saireito on the Activity of Mucosal-Type Mast Cells
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DOI：
10.1248/bpb.33.142
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发表时间：
2010-01-01
影响因子：
2
通讯作者：
Kadowaki, Makoto
中科院分区：
文献类型：
--
作者：
Kageyama-Yahara, Natsuko;Wang, Ping;Kadowaki, Makoto
据推测，溃疡性结肠炎 (UC) 中的粘膜炎症主要由 2 型 T 辅助细胞免疫反应调节，结肠中的粘膜肥大细胞被认为在粘膜炎症的发病机制中发挥着重要作用。 Saireito是一种质量标准化的日本草药，起源于传统中药（汉方药），由两种不同的汉方药（正斋草和高丽散）组成，在日本常用于治疗UC。在这项研究中，我们检查了这些汉方药物及其成分对抗原诱导的粘膜型肥大细胞脱颗粒的直接影响。用这些药物预处理粘膜型鼠骨髓源性肥大细胞 (mBMMC) 24 小时，并通过 β-己糖胺酶释放评估免疫球蛋白 E (IgE) 受体触发的 mBMMC 脱颗粒。 Goreisan 对 mBMMC 脱粒具有剂量依赖性的抑制作用。五味药中茯苓、猪苓对mBMMCs有抑制作用。麦角甾醇是茯苓和猪苓的主要和常见成分，也抑制 mBMMC 的脱颗粒。我们的结果为解释 saireito 对 UC 的部分有益治疗特性提供了分子基础。
Mucosal inflammation in ulcerative colitis (UC) is presumed to be regulated primarily by type 2 T helper cell immune responses and mucosal mast cells in the colon are thought to play an important role in the pathogenesis of the mucosal inflammation. Saireito, a Japanese herbal medicine of standardized quality, originating from traditional Chinese medicine (Kampo medicine), is composed of two different Kampo medicines (shosaikoto and goreisan) and is often used for UC in Japan. In this study, we examined the direct effects of these Kampo medicines and their constituents on the antigen-induced degranulation of mucosal-type mast cells. Mucosal-type murine bone marrow-derived mast cells (mBMMCs) were pretreated by these drugs for 24 h, and immunoglobulin E (IgE) receptor-triggered degranulation of mBMMCs was assessed by beta-hexosaminidase release. Goreisan showed inhibitory effects on degranulation of mBMMCs in a dose-dependent manner. Among the five constituent medicinal herbs of goreisan, Poria and Polyporus had the inhibitory effects on mBMMCs. Ergosterol, a principal and common component of Poria and Polyporus, also suppressed the degranulation of mBMMCs. Our results provide a molecular basis to explain a portion of the beneficial therapeutic properties of saireito on UC.