Cytology of Vicia species. X. Karyotype evolution and phylogenetic implication in Vicia species of the sections Atossa, Microcarinae, Wiggersia and Vicia
Cytology of Vicia species. X. Karyotype evolution and phylogenetic implication in Vicia species of the sections Atossa, Microcarinae, Wiggersia and Vicia
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DOI：
10.1007/s00709-010-0232-7
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发表时间：
2011-10-01
期刊：
影响因子：
2.9
通讯作者：
Cremonini, R.
中科院分区：
文献类型：
--
作者：
Castiglione, M. Ruffini;Frediani, M.;Cremonini, R.
对属于阿托萨组（Atossa）、小果野豌豆组（Microcarinae）、维格斯组（Wiggersia）和野豌豆组（Vicia）的物种进行了自动核型分析和核糖体DNA间隔区序列分析。基于Rec、Syi和TF%指数确定了核形态学参数，并证明就对称性而言，山黧豆状野豌豆（Vicia lathyroides）的核型是最不对称的。除了对称性指数外，还使用34个核型学参数进行了多变量分析，相应的连锁距离树状图显示出六个不同的组。通过使用内转录间隔区（ITS）DNA序列对所研究的包含组进行分子研究，显示出一种不同的亲缘关系模式。将分子数据集与核型学参数相结合的分支分析表明，野豌豆组和阿托萨组的物种在一个并系群中彼此紧密相连，该并系群包括单系的维格斯组。因此，我们的核型学和分子数据提供了有关所分析物种在野豌豆亚属内系统发育位置的信息，并结合先前通过形态学、同工酶和核糖体基因分析获得的结果进行了讨论。
Automated karyotype analyses and sequence of rDNA spacers have been analysed for the species belonging to sections Atossa, Microcarinae, Wiggersia and Vicia. Karyomorphological parameters, based on Rec, Syi and TF% indices, have been determined and evidenced that, in term of symmetry, the karyotype of Vicia lathyroides was the most asymmetric one. A multivariate analysis using 34 karyological parameters, in addition to the symmetry indices, has been carried out and the corresponding dendrogram of linkage distances showed six different groups. Molecular investigations on the inclusive group in study by employing ITS DNA sequences indicated a different pattern of relationships. The cladistic analysis combining the molecular data set with karyological parameters evidenced that the species of sections Vicia and Atossa join closely to each other in a paraphyletic group, which includes the monophyletic section Wiggersia. Therefore, our karyological and molecular data provide information about the phylogenetic position of the analysed species inside the subgenus Vicia and are discussed in relation to previous results obtained by morphology, isozymes and ribosomal genes analyses.