Interaction between Ataxin-2 Binding Protein 1 and Cubitus-interruptus during wing development in Drosophila.
Interaction between Ataxin-2 Binding Protein 1 and Cubitus-interruptus during wing development in Drosophila.
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DOI：
10.1016/j.ydbio.2010.02.039
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发表时间：
2010-05
影响因子：
2.7
通讯作者：
N. Usha;L. Shashidhara
中科院分区：
文献类型：
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作者：
N. Usha;L. Shashidhara
动物的生长和发育依赖于关键信号通路的重复使用，例如刺猬 (Hh) 通路。人们普遍认为 Cubitus-interruptus (Ci) 介导 Hh 通路的所有功能。在这里，我们报道了 CG32062，Ataxin-2 结合蛋白 1 (dA2BP1) 的果蝇同源物，作为 Ci 的辅助因子，指定成虫翅膀 L3 和 L4 静脉之间的静脉区域。具体来说，在发育中的翼成虫盘的该区域中，Ci 介导的结/科利尔 (kn) 反式激活依赖于 dA2BP1 功能。蛋白质相互作用研究和染色质免疫沉淀实验表明，Ci 帮助 dA2BP1 结合 kn 启动子，这反过来可能帮助 Ci 激活 kn 表达。这些结果表明 Ci 可能激活 kn 等靶标的机制，而 kn 不具有经典的 Ci/Gli 结合位点。
Animal growth and development is dependent on reiterative use of key signaling pathways such as Hedgehog (Hh) pathway. It is widely believed that Cubitus-interruptus (Ci) mediates all functions of Hh pathway. Here we report that CG32062, the Drosophila homologue of Ataxin-2 Binding Protein 1 (dA2BP1), functions as a cofactor of Ci to specify intervein region between L3 and L4 veins of the adult wing. Specifically, Ci-mediated transactivation of knot/collier (kn) in this region of the developing wing imaginal disc is dependent on dA2BP1 function. Protein interaction studies and chromatin-immunoprecipiation experiments suggest that Ci helps dA2BP1 to bind kn promoter, which in turn may help Ci to activate kn expression. These results suggest a mechanism by which Ci may activate targets such as kn, which do not have classical Ci/Gli-binding sites.