Spiroplasma Isolated From Third-Generation Laboratory Colony Ixodes persulcatus Ticks.
Spiroplasma Isolated From Third-Generation Laboratory Colony Ixodes persulcatus Ticks.
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从第三代实验室菌落ixodes persulcatus tick虫中分离出来的螺旋藻。
DOI：
10.3389/fvets.2021.659786
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发表时间：
2021
影响因子：
3.2
通讯作者：
Bell-Sakyi L
中科院分区：
文献类型：
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作者：
Beliavskaia A;Hönig V;Erhart J;Vyhlidalova T;Palus M;Cerny J;Kozlova I;Ruzek D;Palomar AM;Bell-Sakyi L
螺原体是蜱和其他节肢动物的垂直传播的内共生体。据报道，现场采集的硬蜱含有螺原体，但对于该蜱种在实验室定殖期间的持久性尚不清楚。我们成功地将螺原体从 6/10 未喂食的成年蜱（属于第三代 I. persulcatus 实验室群体）的内脏中分离到蜱细胞培养物中。我们通过对 16S rRNA 和 rpoB 基因片段进行属特异性 PCR 扩增，进一步筛选了来自同一群体的 51 只成年雄性和雌性蜱虫是否存在螺原体；这些蜱虫 100% 被感染，并且 16S rRNA 序列与先前发表的从现场采集的 I. persulcatus 中分离出的螺原体序列显示出 99.8% 的相似性。我们的研究表明，螺原体内共生体在定植的 I. persulcatus 中持续存在至少三代，并证实了蜱细胞系用于分离和培养这种细菌的有用性。
Spiroplasma are vertically-transmitted endosymbionts of ticks and other arthropods. Field-collected Ixodes persulcatus have been reported to harbour Spiroplasma, but nothing is known about their persistence during laboratory colonisation of this tick species. We successfully isolated Spiroplasma from internal organs of 6/10 unfed adult ticks, belonging to the third generation of an I. persulcatus laboratory colony, into tick cell culture. We screened a further 51 adult male and female ticks from the same colony for presence of Spiroplasma by genus-specific PCR amplification of fragments of the 16S rRNA and rpoB genes; 100% of these ticks were infected and the 16S rRNA sequence showed 99.8% similarity to that of a previously-published Spiroplasma isolated from field-collected I. persulcatus. Our study shows that Spiroplasma endosymbionts persist at high prevalence in colonised I. persulcatus through at least three generations, and confirms the usefulness of tick cell lines for isolation and cultivation of this bacterium.