Epigenome analysis of pluripotent stem cells.
Epigenome analysis of pluripotent stem cells.
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DOI：
10.1007/978-1-62703-348-0_16
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Hart, Ronald P
中科院分区：
文献类型：
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作者：
Ricupero, Christopher L;Swerdel, Mavis R;Hart, Ronald P
Mis-regulation of gene expression due to epigenetic abnormalities has been linked with complex genetic disorders, psychiatric illness and cancer. In addition, the dynamic epigenetic changes that occur in pluripotent stem cells are believed to impact regulatory networks essential for proper lineage development. Chromatin immunoprecipitation (ChIP) is a technique used to isolate and enrich chromatin fragments using antibodies against specific chromatin modifications, such as DNA binding proteins or covalent histone modifications. Until recently, many ChIP protocols required millions of cells for each immunoprecipitation. This severely limited analysis of rare cell populations or post-mitotic, differentiated cell lines. Here, we describe a low cell number ChIP protocol with next generation sequencing and analysis, that has the potential to uncover novel epigenetic regulatory pathways that were previously difficult or impossible to obtain.
