Biologic and genetic comparison of Toxoplasma gondii isolates in free-range chickens from the northern Para state and the southern state Rio Grande do Sul, Brazil revealed highly diverse and distinct parasite populations
Biologic and genetic comparison of Toxoplasma gondii isolates in free-range chickens from the northern Para state and the southern state Rio Grande do Sul, Brazil revealed highly diverse and distinct parasite populations
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DOI：
10.1016/j.vetpar.2006.08.024
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发表时间：
2007-01-31
影响因子：
2.6
通讯作者：
Su, C.
中科院分区：
文献类型：
--
作者：
Dubey, J. P.;Sundar, N.;Su, C.
对来自南美洲巴西北方帕拉州和南方南里奥格兰德州的84只散养鸡进行了弓形虫感染率测定。用改良凝集试验（MAT）检测了84只鸡的弓形虫抗体，阳性率为46.4%（39/84），抗体滴度分别为1：10、1：20、1：40、1：80、1：160、1：320、1：640和≥ 1：1280。将滴度为1：20或更低的45只鸡的心脏和脑合并，并喂食两只无刚地弓形虫的猫。对39只效价在1：10以上的鸡的心和脑进行了小鼠生物测定。检查猫粪便中的卵囊。一只猫喂食来自南里奥格兰德的31只鸡的组织，这些鸡的滴度低于1：10。用生物测定法从33只鸡体内分离出弓形虫，其MAT滴度在1：20或更高。10个分离株感染小鼠全部死于弓形虫病。用SAG 1、SAG 2、SAG 3、BTUB、GRA 6、c22-8、c29-2、L358、PK 1、一个新的SAG 2和Apico等11个遗传标记对34株（帕拉15株，南里奥格兰德19株）进行基因分型。帕拉分离株有11种基因型，南里奥格兰德有7种基因型。两个地理位置之间没有共享基因型。这些数据表明，弓形虫分离株是高度多样性和遗传不同的两个不同的地区在巴西，相距3500公里。(c)2006 Elsevier B. V.保留所有权利。
The prevalence of Toxoplasma gondii in 84 free-range chickens (34 from the northern Para state, and 50 from Rio Grande do Sul, the southern state) from Brazil, South America was determined. Antibodies to T gondii were assayed by the modified agglutination test (MAT), and found in 39 (46.4%) of 84 chickens with titers of 1: 10 in one, 1:20 in two, 1:40 in four, 1:80 in seven, 1: 160 in five, 1:320 in six, 1:640 in eight and >= 1: 1280 in six. Hearts and brains of 45 chickens with titers of 1:20 or less were pooled and fed to two T gondii-free cats. Hearts and brains of 39 chickens with titers of 1: 10 or higher were bioassayed in mice. Feces of cats were examined for oocysts. One cat fed tissues from 31 chickens with titers of less than 1:10 from Rio Grande do Sul shed T gondii oocysts. T gondii was isolated by bioassay in mice from 33 chickens with MAT titers of 1:20 or higher. All infected mice from 10 isolates died of toxoplasmosis. All 34 isolates (15 from Para, 19 from Rio Grande do Sul) were genotyped using 11 genetic markers including SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, a new SAG2 and Apico. Eleven genotypes were revealed for Para isolates and seven genotypes for Rio Grande do Sul. No genotype was shared between the two geographical locations. These data suggest that T gondii isolates are highly diverse and genetically distinct between the two different regions in Brazil that are 3500 km apart. (c) 2006 Elsevier B.V. All rights reserved.