Inhibition of UV-induced DNA Damage of Cells by Hydrostatic Pressure
Inhibition of UV-induced DNA Damage of Cells by Hydrostatic Pressure
复制标题
静水压抑制紫外线诱导的细胞 DNA 损伤
DOI：
10.11395/jjsem.21.208
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
長山 和亮
中科院分区：
文献类型：
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作者：
上杉 薫;佐藤 賢也;長山 和亮
Kaoru Uesugi、Kenia Sato和Kazuaki Nagayama细胞受到各种机械刺激的影响，改变了它们的机械和生理特性。最近，我们发现机械应力可能抑制紫外线(UV)诱导的细胞DNA损伤。因此，本研究对静水压力作用下培养细胞的形态变化和核DNA的紫外线损伤进行了研究。首先，建立了静水加压实验装置，观察了大鼠血管平滑肌细胞(A7r5)细胞核的形态变化。结果表明，静水压力作用下，细胞胞核形状变圆，投射面积和体积减小。细胞核高度无明显变化。然后，评价静水压力对A7r5细胞核DNA的紫外线损伤作用。γ-H_2AX对细胞DNA损伤的评价表明，静水压对细胞的紫外线损伤有抑制作用。核染色法评价表明，暴露于静水压力下的细胞的紫外线损伤比未暴露的细胞要小得多，同时还伴随着核内DNA聚集。这些结果表明，由于核形状的减少而发生的DNA聚集可能是抵御紫外线照射的物理屏障。
Kaoru UESUGI, Kenya SATO and Kazuaki NAGAYAMA Cells are affected by various mechanical stimulations and change their mechanical and physiological properties. Recently, we found that mechanical stress possibly inhibits the ultraviolet (UV)-induced DNA damage of cells. Thus, in this study, the morphological change and the UV damage of nuclear DNA of cultured cells exposed to hydrostatic pressure were evaluated. First, a hydrostatic pressure-imposing experimental setup was constructed and morphological change of rat smooth muscle cell’s (A7r5) nucleus was evaluated. The results showed that the shape of the nucleus of cells exposed to hydrostatic pressure came close circle and the projected area and the volume decreased. The height of the nucleus did not change significantly. Then, the UV damage of nuclear DNA of A7r5 cells exposed to hydrostatic pressure was evaluated. An evaluation of breakage of DNA with γ-H2AX shown that the UV damage of cells exposed to hydrostatic pressure was inhibited. An evaluation using nuclear staining was shown that the UV damage of cells exposed to hydrostatic pressure was more decreased than that of cells unexposed, simultaneously with intranuclear DNA aggregations. These results mean that the DNA aggregation that occurred by a decrease of nuclear shape may work as a physical barrier against UV irradiation.