IDENTIFICATION AND CHARACTERIZATION OF CONSERVED AND VARIABLE REGIONS IN THE ENVELOPE GENE OF HTLV-III LAV, THE RETROVIRUS OF AIDS
IDENTIFICATION AND CHARACTERIZATION OF CONSERVED AND VARIABLE REGIONS IN THE ENVELOPE GENE OF HTLV-III LAV, THE RETROVIRUS OF AIDS
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DOI：
10.1016/0092-8674(86)90778-6
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发表时间：
1986-06-06
期刊：
影响因子：
64.5
通讯作者：
WONGSTAAL, F
中科院分区：
文献类型：
--
作者：
STARCICH, BR;HAHN, BH;WONGSTAAL, F
为了确定HTLV-III/LAV独立分离株中存在的遗传变异的程度和性质，测定了两种AIDS病毒的整个包膜基因和部分gag和pol的核苷酸序列。结果表明，整个病毒基因组的变异是广泛的，特别是包膜基因是最高度可变的。在包膜内，变化在细胞外区域内最普遍，其中聚集的核苷酸取代和缺失/插入是明显的。基于预测的二级蛋白质结构和亲水性，这些高变区代表潜在的抗原位点。与高变区相反，细胞外包膜和整个包膜结构（包括18个半胱氨酸残基中的18个）以及大部分跨膜区中的其他序列高度保守。
To determine the extent and nature of genetic variation present in independent isolates of HTLV-III/LAV, the nucleotide sequences of the entire envelope gene and parts of a gag and pol were determined for two AIDS viruses. The results indicated that variation throughout the viral genome is extensive and that the envelope gene in particlar is most highly variable. Within the envelope, changes were most prevalent within the extracellular region where clustered nucleotide substitutions and deletions/insertions were evident. Based on predicted secondary protein structure and hydrophilicity, these hypervariable regions represent potential antigenic sites. In contrast to the hypervariable regions, other sequences in the extracellular envelope and the overall envelope structure (including 18 of 18 cysteine residues), as well as most of the transmembrane region, were highly conserved.