Solitary chemoreceptor cell proliferation in adult nasal epithelium
Solitary chemoreceptor cell proliferation in adult nasal epithelium
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DOI：
10.1007/s11068-005-5051-y
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发表时间：
2005-03-01
期刊：
影响因子：
--
通讯作者：
Finger, TE
中科院分区：
文献类型：
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作者：
Gulbransen, BD;Finger, TE
小鼠和大鼠中的鼻三叉神经化学敏感性部分由鼻上皮中的孤立化学感受器细胞（SCC）介导（Finger等人，2003年）。许多鼻SCC表达G蛋白α-味觉调节蛋白以及味觉信号级联的其他元件，包括磷脂酶C β 2、TRPM 5和T2 R味觉受体。虽然一些感觉细胞群体在整个生命过程中会被替换（味觉和嗅觉），但其他感觉细胞群体则不会（毛细胞和颈动脉体化学感受器）。这些实验旨在测试成年小鼠的上皮内是否产生新的SCC。向野生型C57/B6小鼠注射胸苷类似物5-溴-2 '-脱氧尿苷（BrdU）以标记分裂细胞。在注射后的不同时间（1-40天），用4%多聚甲醛灌注小鼠并准备用于双标记免疫细胞化学。早在BrdU注射后3天就检测到双标记细胞，并在注射后保持长达12天，这表明SCC确实像周围的鼻上皮一样经历了更新。在24天后未检测到BrdU标记的细胞，表明SCC的相对快速的替换。
Nasal trigeminal chemosensitivity in mice and rats is mediated in part by solitary chemoreceptor cells (SCCs) in the nasal epithelium (Finger et al., 2003). Many nasal SCCs express the G-protein a-gustducin as well as other elements of the bitter-taste signaling cascade including phospholipase C beta 2, TRPM5 and T2R bitter-taste receptors. While some populations of sensory cells are replaced throughout life (taste and olfaction), others are not (hair cells and carotid body chemoreceptors). These experiments were designed to test whether new SCCs are generated within the epithelium of adult mice. Wild type C57/B6 mice were injected with the thymidine analog 5-bromo-2'-deoxyuridine (BrdU) to label dividing cells. At various times after injection (1-40 days), the mice were perfused with 4% paraformaldehyde and prepared for dual-label immunocytochemistry. Double labeled cells were detected as early as 3 days post BrdU injection and remained for as long as 12 days post-injection suggesting that SCCs do undergo turnover like the surrounding nasal epithelium. No BrdU labeled cells were detected after 24 days suggesting relatively rapid replacement of the SCCs.