Enzymatic excision of free hypoxanthine from polydeoxynucleotides and DNA containing deoxyinosine monophosphate residues.
Enzymatic excision of free hypoxanthine from polydeoxynucleotides and DNA containing deoxyinosine monophosphate residues.
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从含有脱氧肌苷单磷酸残基的聚脱氧核苷酸和 DNA 中酶切游离次黄嘌呤。
DOI：
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发表时间：
1978
影响因子：
4.8
通讯作者：
T. Lindahl
中科院分区：
文献类型：
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作者：
P. Karran;T. Lindahl
大肠杆菌细胞提取物中存在一种DNA糖基化酶，通过选择性切割多脱氧核苷酸和含有该非常规核苷酸的DNA中的dIMP残基释放游离次黄嘌呤。部分纯化的酶，称为次黄嘌呤-DNA糖基化酶，不需要二价阳离子或磷酸盐的活性，它的行动更有效地对双链比单链底物。该酶具有不同于尿嘧啶-DNA糖基化酶或3-甲基腺嘌呤-DNA糖基化酶的性质，并以正常水平存在于E.大肠杆菌突变体缺乏后两种DNA糖基化酶。
A DNA glycosylase that releases free hypoxanthine by selective cleavage of dIMP residues in polydeoxynucleotides and DNA containing this nonconventional nucleotide is present in Escherichia coli cell extracts. The partly purified enzyme, termed hypoxanthine-DNA glycosylase, does not require divalent cations or phosphate for activity, and it acts more efficiently on double-stranded than on single-stranded substrates. The enzyme has properties different from either uracil-DNA glycosylase or 3-methyladenine-DNA glycosylase and is present at normal levels in E. coli mutants deficient in either of the latter two DNA glycosylases.