Acceleration of corneal wound healing in diabetic rats by the antioxidant trolox.
Acceleration of corneal wound healing in diabetic rats by the antioxidant trolox.
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发表时间：
1996-07
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通讯作者：
C. Hallberg;Stefan D. Trocme;Naseem H. Ansari
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作者：
C. Hallberg;Stefan D. Trocme;Naseem H. Ansari
据报道，长期糖尿病患者存在多种角膜并发症，但其确切发病机制尚不清楚。据观察，与正常动物相比，糖尿病大鼠的上皮伤口愈合速度延迟。在这里，我们介绍了自由基清除剂 Trolox（一种水溶性维生素 E 类似物）对糖尿病大鼠角膜上皮伤口愈合的影响。包括三组大鼠：1）正常，2）糖尿病，3）糖尿病+Trolox。 3个月后，处死大鼠并摘除角膜。制作标准3毫米直径的角膜上皮缺损，并在无菌细胞培养箱中于37℃培养18小时后测量残留的上皮缺损。以mm2 测量的伤口愈合数据用于统计分析。与对照组相比，糖尿病患者角膜上皮缺损明显更大（p < 0.05）。与未经治疗的糖尿病大鼠相比，用 Trolox 抗氧化剂治疗糖尿病大鼠产生的上皮缺损明显较小（p < 0.05）。这些研究表明自由基参与了糖尿病患者角膜上皮伤口愈合的延迟。
Several corneal complications have been reported in patients with long standing diabetes, but their exact pathogenesis is not well understood. It has been observed that the rate of epithelial wound healing in diabetic rats is delayed compared to those in normal animals. Here we present the effect of the free radial scavenger, Trolox, a water soluble vitamin E analogue, on epithelial wound healing in diabetic rat cornea. Three groups of rats were included: 1) normal, 2) diabetic, 3) diabetic + Trolox. After 3 months, rats were sacrificed and corneas removed. Standard 3 mm diameter corneal epithelial defects were made and residual epithelial defects were measured after 18 hours at 37 degrees C in a sterile cell culture incubator. Wound healing data measured in mm2 was used for statistical analysis. There were significantly larger (p < 0.05) epithelial defects in diabetic corneas as compared to control. Treatment with Trolox antioxidant in diabetic rats produced a significantly smaller (p < 0.05) epithelial defect than that of untreated diabetic rats. These studies suggest the involvement of free radicals in the delay of corneal epithelial wound healing in diabetes.