Molecular systematics of Trilliaceae I.: Phylogenetic analyses of Trillium using matK gene sequences
Molecular systematics of Trilliaceae I.: Phylogenetic analyses of Trillium using matK gene sequences
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DOI：
10.1007/pl00013853
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发表时间：
1999-03-01
影响因子：
2.8
通讯作者：
Kawano, S
中科院分区：
文献类型：
--
作者：
Osaloo, SK;Utech, FH;Kawano, S
使用 41 个延龄草类群和两个外群类群（Veratrum maackii 和 Helonias bullata）对叶绿体基因 matK 进行了比较 DNA 测序。共对1608个碱基对进行分析比较，得出延龄草属物种间共有61个可变位点（36个信息量）。还检测到 6 个或 15 个碱基对的 15 个插入/缺失事件 (indel)。序列数据的系统发育分析表明，Phyllantherum 亚属（无梗花物种）形成了一个独特的单系群，而延龄草亚属（有花梗花物种）不形成单系群，而是由三个不同的群和三个位于树基部的物种组成：（1）直立群，（2）Grandiflorum群，（3）Pusillum群和（4）三个种，包括 T. govanianum、T. undulatum 和 T.对抗。 T. matche 似乎是群内成员中基础差异最大且非常专业的分类单元。我们的 matK 数据表明，叶下珠亚属的最近亲是 Grandiflorum 组。该结果与cpDNA的RFLP分析结果一致，也与形态性状的分支分析结果基本一致。
Comparative DNA sequencing of the chloroplast gene matK was conducted using 41 Trillium taxa and two outgroup taxa (Veratrum maackii and Helonias bullata). A total of 1608 base pairs were analyzed and compared., and then there were 61 variable (36 informative) sites among Trillium species. Fifteen insertion/deletion events (indels) of six or fifteen base pairs were also detected. Phylogenetic analyses of the sequence data revealed that the subgenus Phyllantherum (sessile-flowered species) forms a distinct monophyletic group, whereas the subgenus Trillium (pedicellate-flowered species) does not form a monophyletic group, and is composed of three distinct groups and three basally located species in the tree: (1) the Erectum group, (2) the Grandiflorum group, and (3) the Pusillum group and (4) the three species, including T. govanianum, T. undulatum, and T. rivale. T. rivale appears to be the most basally diverged and a very specialized taxon among the ingroup members. Our matK data indicated that the closest relative of the subgenus Phyllantherum is the Grandiflorum group. The results are concordant with the results of the RFLP analysis of cpDNA and also more or less with those of the cladistic analysis of morphological characters.