The alteration of gut microbiota in newly diagnosed type 2 diabetic patients
The alteration of gut microbiota in newly diagnosed type 2 diabetic patients
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DOI：
10.1016/j.nut.2018.11.019
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发表时间：
2019-07-01
期刊：
影响因子：
4.4
通讯作者：
Yang, Suh-Ching
中科院分区：
文献类型：
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作者：
Chen, Pei-Chi;Chien, Yi-Wen;Yang, Suh-Ching
目的：肠道微生物群失调与糖尿病有关;然而，以前的研究结果相互矛盾。为了阐明2型糖尿病和肠道菌群之间的关系，本研究分析了粪便肠道菌群的组成及其与新诊断的，未经治疗的糖尿病患者和健康对照组的特定临床参数的相关性。方法：共有50例新诊断的2型糖尿病患者和50名健康对照参与了这项研究。在开始抗糖尿病治疗前和治疗后3个月，采集糖尿病患者的粪便样本、血液样本和食物日记。这些样品也从健康对照中收集。结果：与健康对照组相比，糖尿病患者粪便中乳酸杆菌的数量显著增加，而球形梭菌和纤细梭菌的数量显著减少。乳酸杆菌与血糖、糖化血红蛋白和稳态模型评估呈显著正相关，而C。coccoides和C.瘦素与糖尿病各项指标呈显著负相关。此外，新诊断的糖尿病患者中C的存在显著减少。coccoides和C.结论：治疗3 mo后，粪便中乳酸杆菌、球状梭菌和梭菌的数量与治疗前相比明显减少。2型糖尿病患者与健康对照组间血清瘦素水平有显著性差异。治疗3个月后，糖尿病患者的梭菌水平也发生了显著变化。需要进一步的研究来阐明肠道微生物群失调与2型糖尿病之间的相关性或因果关系。(C)2018爱思唯尔公司All rights reserved.
Objectives: Gut microbiota dysbiosis is known to be associated with diabetes; however, the findings of previous studies are conflicting. To clarify the association between type 2 diabetes and the gut microbiota, the present study analyzed the composition of fecal gut microbiota and its correlation with specific clinical parameters in newly diagnosed, treatment-naive diabetic patients and healthy controls.Methods: A total of 50 patients with newly diagnosed type 2 diabetes and 50 healthy control participants were enrolled in the study. Fecal samples, blood samples, and food diaries were collected from the diabetic patients before and 3 mo after the start of their antidiabetic treatment. These samples were also collected from the healthy controls. The gut microbiota was characterized by 16S ribosomal RNA analysis using quantitative polymerase chain reaction.Results: The fecal count of Lactobacillus was significantly higher, whereas Clostridium coccoides and Clostridium leptum were significantly lower in the diabetic patients compared with the healthy controls. Lactobacillus was significantly positively correlated with glucose, glycated hemoglobin, and the homeostatic model assessment, whereas C. coccoides and C. leptum were significantly negatively correlated with the diabetic parameters. In addition, the newly diagnosed diabetic patients had a significant decrease in the presence of C. coccoides and C. leptum after 3 mo of treatment compared with before treatment.Conclusions: The amount of fecal Lactobacillus, C coccoides, and C. leptum was significantly different between the patients with type 2 diabetes and the healthy controls. The levels of Clostridium were also significantly changed after 3 mo of treatment in the diabetic patients. Further research is needed to clarify the correlation or causal relationship between the gut microbiota dysbiosis and type 2 diabetes. (C) 2018 Elsevier Inc. All rights reserved.