Cytotoxicity of the soluble and insoluble fractions of atmospheric fine particulate matter
Cytotoxicity of the soluble and insoluble fractions of atmospheric fine particulate matter
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大气细颗粒物可溶性和不溶性部分的细胞毒性
DOI：
10.1016/j.jes.2020.01.012
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发表时间：
2020-05-01
影响因子：
6.9
通讯作者：
Jiang, Wei
中科院分区：
文献类型：
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作者：
Liu, Ling;Zhou, Qiuhua;Jiang, Wei
吸入的大气细颗粒物（PM2.5）包括可溶性和不溶性部分，每一部分都能与细胞相互作用并产生不良影响。在中国济南采集PM2.5样品，分离可溶性和不溶性组分。理化性质分析表明，可溶性组分主要含有水溶性离子和有机酸，不溶性组分主要含有高岭石、碳酸钙和少量有机碳。用石英晶体微天平（QCM-D）检测PM2.5与模型细胞膜之间的相互作用，以定量PM2.5在膜上的附着和膜破裂。研究了总PM2.5以及可溶性和不溶性组分的细胞毒性。带负电荷的PM2.5可以粘附在带正电荷的膜上并破坏它们。PM2.5也附着在带负电荷的膜上，但不会导致膜破裂。因此，静电排斥不能阻止PM2.5附着，但静电吸引引起显著的膜破裂。使用人肺上皮细胞系A549进行细胞毒性评估。检测到的膜渗漏、细胞肿胀和起泡指示细胞坏死过程。此外，不溶性PM2.5组分引起更高的细胞死亡率和更严重的细胞膜损伤比可溶性组分。活性氧（ROS）的水平增强的两个馏分没有显着差异。本研究结果为更好地理解PM2.5细胞毒性的机制以及PM2.5溶解性对细胞毒性的影响提供了更多的信息。(C)2020中国科学院生态环境科学研究中心由爱思唯尔公司出版
Inhaled atmospheric fine particulate matter (PM2.5) includes soluble and insoluble fractions, and each fraction can interact with cells and cause adverse effects. PM2.5 samples were collected in Jinan, China, and the soluble and insoluble fractions were separated. According to physiochemical characterization, the soluble fraction mainly contains water-soluble ions and organic acids, and the insoluble fraction mainly contains kaolinite, calcium carbonate and some organic carbon. The interaction between PM2.5 and model cell membranes was examined with a quartz crystal microbalance with dissipation (QCM-D) to quantify PM2.5 attachment on membranes and membrane disruption. The cytotoxicity of the total PM2.5 and the soluble and insoluble fractions, was investigated. Negatively charged PM2.5 can adhere to the positively charged membranes and disrupt them. PM2.5 also adheres to negatively charged membranes but does not cause membrane rupture. Therefore, electrostatic repulsion does not prevent PM2.5 attachment, but electrostatic attraction induces remarkable membrane rupture. The human lung epithelial cell line A549 was used for cytotoxicity assessment. The detected membrane leakage, cellular swelling and blebbing indicated a cell necrosis process. Moreover, the insoluble PM2.5 fraction caused a higher cell mortality and more serious cell membrane damage than the soluble fraction. The levels of reactive oxygen species (ROS) enhanced by the two fractions were not significantly different. The findings provide more information to better understand the mechanism of PM2.5 cytotoxicity and the effect of PM2.5 solubility on cytotoxicity. (C) 2020 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.