A dopamine- and cyclic AMP-regulated phosphoprotein enriched in dopamine-innervated brain regions
A dopamine- and cyclic AMP-regulated phosphoprotein enriched in dopamine-innervated brain regions
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DOI：
10.1038/301069a0
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发表时间：
1983-01
期刊：
影响因子：
64.8
通讯作者：
S. Walaas;D. Aswad;D. Aswad;P. Greengard
中科院分区：
文献类型：
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作者：
S. Walaas;D. Aswad;D. Aswad;P. Greengard
几种哺乳动物神经递质候选者，例如，多巴胺1，多巴胺2，3和去甲肾上腺素4，5，可能会通过调节蛋白磷酸化系统发挥一些突触作用6，7。比较脑磷蛋白与神经递质系统的区域分布可能有助于确定参与特定神经递质功能的特定磷蛋白。在这里，我们报告了一个这样的磷蛋白与多巴胺通路在大脑中的协会。这种蛋白质的表观分子量（MW）为32，000（32 K），似乎只存在于神经组织中。其在脑内的区域分布与含多巴胺的神经末梢的模式非常相似;更具体地说，该蛋白似乎富集在具有D-1受体（与腺苷酸环化酶偶联的多巴胺受体8）的多巴胺感受神经元中。这些多巴胺感受神经元中蛋白质的磷酸化状态可由多巴胺和环AMP调节。这些结果表明，磷蛋白可能介导的多巴胺作用于多巴胺感受神经元的某些跨突触效应。
Several mammalian neurotransmitter candidates, for example, serotonin1, dopamine2,3and noradrenaline4,5, may exert some of their synaptic effects by regulating protein phosphorylation systems6,7. Comparison of the regional distribution of brain phosphoproteins with neurotransmitter systems may help to identify the specific phosphoproteins involved in the functions of particular neurotransmitters. Here we report the association of one such phosphoprotein with the dopamine pathways in brain. This protein, of apparent molecular weight (MW) 32,000 (32K), seems to be present only in nervous tissue. Its regional distribution within the brain is very similar to the pattern of dopamine-containing nerve terminals; more specifically, the protein appears to be enriched in those dopaminoceptive neurones which possess D-1 receptors (dopamine receptors coupled to adenylate cyclase8). The state of phosphorylation of the protein in these dopaminoceptive neurones can be regulated by both dopamine and cyclic AMP. These results suggest that the phosphoprotein may mediate certain of the transsynaptic effects of dopamine acting on dopaminoceptive neurones.