RPRD Proteins Control Transcription in Human Cells
RPRD Proteins Control Transcription in Human Cells
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DOI：
10.1101/2021.06.20.449126
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发表时间：
2021-06
期刊：
影响因子：
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通讯作者：
Kinga Winczura;Hurmuz Ceylan;M. Sledziowska;Matt Jones;Holly Fagarasan;Jianming Wang;Marco Saponaro;R. Arnold;D. Hebenstreit;Pawel Grzechnik
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文献类型：
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作者：
Kinga Winczura;Hurmuz Ceylan;M. Sledziowska;Matt Jones;Holly Fagarasan;Jianming Wang;Marco Saponaro;R. Arnold;D. Hebenstreit;Pawel Grzechnik
转录调控是一个重要的过程，允许细胞对各种内部和外部信号做出反应。RNA聚合酶II（Pol II）的活性受许多因子控制，这些因子与其最大亚基RPB 1的C-末端结构域（CTD）结合，并刺激或抑制RNA合成。在这里，我们证明了CTD相互作用蛋白，RPRD 2，RPRD 1B和RPRD 1A作为转录的负调控因子，其水平与人类细胞中新生和新转录的RNA的积累呈负相关。我们发现，RPRD蛋白形成相互排斥的复合物与Pol II协调其在转录控制中的作用。我们的数据表明，RPRD 2对转录产生最大的影响，并有可能改变关键的生物学过程，包括细胞应激反应和细胞生长。
The regulation of transcription is an essential process that allows the cell to respond to various internal and external signals. RNA Polymerase II (Pol II) activity is controlled by a number of factors which bind to the C-terminal domain (CTD) of its largest subunit, RPB1, and stimulate or suppress RNA synthesis. Here, we demonstrate that CTD-interacting proteins, RPRD2, RPRD1B and RPRD1A act as negative regulators of transcription and their levels inversely correlate with the accumulation of nascent and newly transcribed RNA in human cells. We show that the RPRD proteins form mutually exclusive complexes with Pol II to coordinate their roles in transcriptional control. Our data indicate that RPRD2 exerts the most substantial impact on transcription and has the potential to alter key biological processes including the cellular stress response and cell growth.