Differential monocytic cell adherence to specific anatomic regions of the canine aorta.
Differential monocytic cell adherence to specific anatomic regions of the canine aorta.
复制标题
单核细胞粘附到犬主动脉特定解剖区域的差异。
DOI：
10.1159/000159101
复制
发表时间：
1995
影响因子：
1.7
通讯作者：
Graham,LM
中科院分区：
文献类型：
--
作者：
Margolin,DA;Madura,JA;delaMotte,CA;Fox,PL;DiCorleto,PE;Graham,LM
循环单核细胞和血管内皮细胞（EC）以复杂和动态的方式相互作用，在血管床之间变化。本研究的目的有两个：确定单核细胞对血管内皮的粘附在犬主动脉的特定解剖区域之间是否存在差异，并研究已知的EC刺激剂对单核细胞对这些区域细胞粘附的影响。最初的体外研究测量了人单核细胞系U937细胞对犬颈静脉和主动脉EC单层的粘附性，结果显示，白细胞介素-1 （IL-1）、脂多糖（LPS）、12-肉豆酸酯13-醋酸酯（PMA）或凝血酶对EC的粘附性有剂量依赖性的增加。虽然在组织培养中，单核细胞对未刺激EC的粘附没有区域差异，但研究表明，远端主动脉培养的单核细胞对刺激EC的粘附比近端主动脉培养的单核细胞更强。在器官培养中，未刺激的U937细胞或自体单核细胞对远端主动脉的粘附明显大于近端主动脉。尽管单核细胞对近端和远端主动脉的粘附都随着刺激而增加，但近端主动脉的百分比增加（IL-1增加1086%，PMA增加237%，LPS增加209%，凝血酶增加174%）大于远端主动脉，这表明来自单个血管的不同解剖区域的内皮具有显著的功能差异。这可能与血管疾病的区域特异性有直接关系。
Circulating monocytes and vascular endothelial cells (EC) interact in a complex and dynamic manner that varies between vascular beds. The objective of this study was twofold: to ascertain if monocytic cell adhesion to vascular endothelium differed between specific anatomic regions of the canine aorta, and to investigate the effect of known EC stimulators on monocytic cell adhesion to cells from these regions. Initial in vitro studies measuring adherence of U937 cells, a human monocytic cell line, to canine jugular vein and aortic EC monolayers revealed a dose-dependent increase in adhesion to EC stimulated with interleukin-1 (IL-1), lipopolysaccharide (LPS), phorbol 12-myristate 13-acetate (PMA), or thrombin. While there was no regional difference in monocytic cell adherence to unstimulated EC in tissue culture, studies demonstrated greater monocytic cell adhesion to stimulated EC cultured from the distal versus proximal aorta. In organ culture, unstimulated adhesion of U937 cells or autologous monocytes was significantly greater to the distal aorta than the proximal aorta. Although monocytic cell adhesion to both the proximal and distal aorta increased with stimulation, the percentage increase in the proximal aorta, 1,086% with IL-1, 237% with PMA, 209% with LPS, and 174% with thrombin, was greater than in the distal aorta, demonstrating a significant functional difference in the endothelium from separate anatomic regions of a single vessel. This may have a direct relevance to the regional specificity of vascular disease.