Diagnostic potential of fractionated Brugia malayi microfilarial excretory/secretory antigen for bancroftian filariasis
Diagnostic potential of fractionated Brugia malayi microfilarial excretory/secretory antigen for bancroftian filariasis
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DOI：
10.1016/s0035-9203(96)90236-9
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发表时间：
1996-05-01
影响因子：
2.2
通讯作者：
Harinath, BC
中科院分区：
文献类型：
--
作者：
Chenthamarakshan, V;Reddy, MVR;Harinath, BC
由于没有足够的寄生虫材料从班氏丝虫，异源抗原从马来丝虫已被调查用于诊断班氏丝虫病。B。马来微丝蚴排泄/分泌抗原（BmmfES）能有效抑制微丝蚴血症者循环抗原组分CFA（2）-1，9，11和12的结合，并能有效抑制微丝蚴血症者循环抗原组分CFA（2）-1，9，11和12的结合，也能有效抑制微丝蚴血症者循环抗原组分CFA（2）-1，9，11和12的结合。班氏微丝蚴排泄/分泌抗原与抗丝虫血清免疫球蛋白的免疫球蛋白G（IgG）级分。BmmfES通过离子交换色谱法在DEAE纤维素上分离成2个级分，BmE DE 1和BmE DE 2。BmE DE 1在检测人血清中丝虫IgG抗体方面略上级全BmmfES和BmE DE 2。对230份不同人群的血清进行BmE DE 1的检测，结果表明，在83%的微丝蚴血症者血清、83%的临床丝虫病患者血清、17%的流行区正常人血清中检出特异性IgG，而在非流行区人群血清中未检出。使用BmE DE 1的检测系统，灵敏度为83%，特异性为83%，在检测微丝蚴血症，特别是在活跃的临床感染，寄生虫通常是看不到的，应该是非常有用的。
Due to the non-availability of sufficient parasite material from Wuchereria bancrofti, a heterologous filarial antigen from Brugia malayi has been investigated for the diagnosis of bancroftian filariasis. B. malayi microfilarial excretory/secretory antigen (BmmfES) effectively inhibited the binding of circulating filarial antigen fractions (CFA(2)-1, 9, 11 and 12) from microfilaraemic cases, and of W. bancrofti microfilarial excretory/secretory antigen, to the immunoglobulin G (IgG) fraction of anti-filarial serum immunoglobulins. BmmfES was separated by ion-exchange chromatography on DEAE cellulose into 2 fractions, BmE DE1 and BmE DE2. BmE DE1 was marginally superior to whole BmmfES and BmE DE2 in detecting filarial IgG antibodies in human sera. 230 human sera from different groups of patients were screened against BmE DE1, which detected specific IgG in 83% of sera from microfilaraemic donors, 83% of sera from patients with clinical filariasis, 17% of sera from normal residents of an endemic area, and in none of the sera from persons living in a non-endemic area. The assay system using BmE DE1, with a sensitivity of 83% and specificity of 83%, should be very useful in detecting microfilaraemia, particularly in active clinical infections where the parasite is usually not seen.