AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis cullin AtCUL1 is required for auxin response
AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis cullin AtCUL1 is required for auxin response
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DOI：
10.1105/tpc.010282
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发表时间：
2002-02-01
期刊：
影响因子：
11.6
通讯作者：
Estelle, M
中科院分区：
文献类型：
--
作者：
del Pozo, JC;Dharmasiri, S;Estelle, M
AXR 1基因的突变导致生长素反应的减少和生长素调节的生长和发育的各种缺陷。在先前的研究中，我们发现AXR 1与ECR 1蛋白形成异源二聚体。这种酶在体外激活泛素相关蛋白RUB 1。此外，我们发现，Skp 1-Cul 1/Cdc 53-F-box（SCF）亚基AtCUL 1在体内被RUB 1修饰。在这份报告中，我们证明了RUB-AtCUL 1的形成是依赖于AXR 1和ECR 1在体内。AXR 1和ECR 1的表达仅限于活跃的细胞分裂和细胞伸长区域，这与它们在生长调节中的作用一致。这些结果为其中AtCULl的RUB缀合影响生长素响应所需的SCF E3的功能的模型提供了强有力的支持。
Mutations in the AXR1 gene result in a reduction in auxin response and diverse defects in auxin-regulated growth and development. In a previous study, we showed that AXR1 forms a heterodimer with the ECR1 protein. This enzyme activates the ubiquitin-related protein RUB1 in vitro. Furthermore, we showed that the Skp1-Cul1/Cdc53-F-box (SCF) subunit AtCUL1 is modified by RUB1 in vivo. In this report, we demonstrate that the formation of RUB-AtCUL1 is dependent on AXR1 and ECR1 in vivo. The expression of AXR1 and ECR1 is restricted to zones of active cell division and cell elongation, consistent with their role in growth regulation. These results provide strong support for a model in which RUB conjugation of AtCULl affects the function of SCF E3s that are required for auxin response.