Visualizing plant development and gene expression in three dimensions using optical projection tomography
Visualizing plant development and gene expression in three dimensions using optical projection tomography
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DOI：
10.1105/tpc.106.043042
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发表时间：
2006-09-01
期刊：
影响因子：
11.6
通讯作者：
Coen, Enrico
中科院分区：
文献类型：
--
作者：
Lee, Karen;Avondo, Jerome;Coen, Enrico
要更深入地了解植物生长和发育的机制，需要在不同阶段和规模上的三维(3D)形态和基因活性的定量数据。为了解决这个问题，我们探索了光学投影层析成像(OPT)作为一种从植物标本中获取3D数据的方法。我们表明，OPT可以方便地应用于各种不同尺度的植物材料，包括幼苗、叶片、花、根、种子、胚胎和分生组织。在最高分辨率下，可以在周围植物结构的背景下看到大的单个细胞。对于自然半透明的结构，如牙根，使用OPT进行实时3D成像也是可能的。可以使用标记基因，如β-葡萄糖醛酸酶，或更直接地通过整体原位杂交来显示基因表达的3D结构域。我们还描述了允许以不同方式交互地容易地量化和可视化3D数据的工具和软件。
A deeper understanding of the mechanisms that underlie plant growth and development requires quantitative data on three-dimensional ( 3D) morphology and gene activity at a variety of stages and scales. To address this, we have explored the use of optical projection tomography ( OPT) as a method for capturing 3D data from plant specimens. We show that OPT can be conveniently applied to a wide variety of plant material at a range of scales, including seedlings, leaves, flowers, roots, seeds, embryos, and meristems. At the highest resolution, large individual cells can be seen in the context of the surrounding plant structure. For naturally semitransparent structures, such as roots, live 3D imaging using OPT is also possible. 3D domains of gene expression can be visualized using either marker genes, such as beta-glucuronidase, or more directly by whole-mount in situ hybridization. We also describe tools and software that allow the 3D data to be readily quantified and visualized interactively in different ways.