SECRETION OF GLYCOSYLATED HUMAN ERYTHROPOIETIN FROM YEAST DIRECTED BY THE ALPHA-FACTOR LEADER REGION
SECRETION OF GLYCOSYLATED HUMAN ERYTHROPOIETIN FROM YEAST DIRECTED BY THE ALPHA-FACTOR LEADER REGION
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DOI：
10.1016/0378-1119(89)90102-9
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发表时间：
1989-06-30
期刊：
影响因子：
3.5
通讯作者：
BANKS, AR
中科院分区：
文献类型：
--
作者：
ELLIOTT, S;GIFFIN, J;BANKS, AR
酵母MF.α的前α-因子前导区。用1基因引导人促红细胞生成素(EPO)分泌到培养液中。对表达水平对生物活性EPO分泌的影响的研究表明，有几个限速步骤。这包括由KEX2编码的内切酶处理α-因子-EPO前体蛋白，并通过分泌途径运输该蛋白。转运的限速步骤是在分泌途径的早期，可能是从内质网到高尔基体。
The pre-pro .alpha.-factor leader region of the yeast MF.alpha. 1 gene was used to direct the secretion of the human glycoprotein, erythropoietin (EPO), into the culture medium. An examination of the role of expression level on secretion of biologically active EPO indicated that there are several rate-limiting steps. These include processing of the .ALPHA.-factor-EPO precursor protein by the KEX2-encoded endoproteinase and transport of the protein through the secretory pathway. The rate-limiting steps for transport were early in the secretory pathway, probably from the endoplasmic reticulum to the Golgi apparatus.