Salmonella Typhimurium-specific bacteriophage ΦSH19 and the origins of species specificity in the Vi01-like phage family.
Salmonella Typhimurium-specific bacteriophage ΦSH19 and the origins of species specificity in the Vi01-like phage family.
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DOI：
10.1186/1743-422x-8-498
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发表时间：
2011-11-02
期刊：
影响因子：
4.8
通讯作者：
Connerton IF
中科院分区：
文献类型：
--
作者：
Hooton SP;Timms AR;Rowsell J;Wilson R;Connerton IF
适用于食品中病原体生物控制的噬菌体的全基因组测序是任何基于噬菌体的干预程序的先决条件。涉及猪生产环境中鼠伤寒沙门氏菌生物卫生处理的试验确定了一种这样的候选物，Φ SH 19。对该噬菌体进行测序，并对其157，785 bp的环状dsDNA基因组进行分析，揭示了许多有趣的特征。Φ SH 19是最近提出的Myoviridae Vi 01-like家族的另一个成员，包含S.伤寒特异性Vi 01和志贺氏菌特异性SboM-AG 3。在核苷酸水平上，Φ SH 19与噬菌体Vi 01高度相似（在基因组长度上80-98%成对同一性），主要差异在于与宿主范围确定相关的区域。对Φ SH 19在该区域内编码的蛋白质的分析揭示了一簇三个推定的尾刺。在三个尾刺突中，两个具有与蛋白质的果胶酸裂解酶家族（Tsp 2）和P22尾刺突家族（Tsp 3）相关的蛋白质结构域，其前景是这些能够使沙门氏菌O抗原降解。Vi 01和SboM-AG 3的尾部刺突蛋白被预测含有保守的右手平行β-螺旋结构，但内部蛋白结构域是不同的，允许不同的宿主特异性。尾刺突蛋白模块的添加或交换是Vi 01样噬菌体家族中宿主范围确定的主要贡献者。
Whole genome sequencing of bacteriophages suitable for biocontrol of pathogens in food products is a pre-requisite to any phage-based intervention procedure. Trials involving the biosanitization of Salmonella Typhimurium in the pig production environment identified one such candidate, ΦSH19. This phage was sequenced and analysis of its 157,785 bp circular dsDNA genome revealed a number of interesting features. ΦSH19 constitutes another member of the recently-proposed Myoviridae Vi01-like family of phages, containing S. Typhi-specific Vi01 and Shigella-specific SboM-AG3. At the nucleotide level ΦSH19 is highly similar to phage Vi01 (80-98% pairwise identity over the length of the genome), with the major differences lying in the region associated with host-range determination. Analyses of the proteins encoded within this region by ΦSH19 revealed a cluster of three putative tail spikes. Of the three tail spikes, two have protein domains associated with the pectate lyase family of proteins (Tsp2) and P22 tail spike family (Tsp3) with the prospect that these enable Salmonella O antigen degradation. Tail spike proteins of Vi01 and SboM-AG3 are predicted to contain conserved right-handed parallel β-helical structures but the internal protein domains are varied allowing different host specificities. The addition or exchange of tail spike protein modules is a major contributor to host range determination in the Vi01-like phage family.