Antibiotic effects against periodontal bacteria in organ cultured tissue.
Antibiotic effects against periodontal bacteria in organ cultured tissue.
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DOI：
10.1002/cre2.48
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发表时间：
2017-03
影响因子：
1.8
通讯作者：
Nishimura F
中科院分区：
文献类型：
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作者：
Takeshita M;Haraguchi A;Miura M;Hamachi T;Fukuda T;Sanui T;Takano A;Nishimura F
通过使用物理器械机械减少感染性细菌被认为是牙周病的主要治疗策略;添加抗生素是预防性的。然而，局部抗生素治疗，结合传统的机械清创术，最近已被证明是更有效的牙周炎患者2型糖尿病。这表明，一些细菌可能会侵入发炎的内牙龈上皮，机械清创术本身将无法完全减少这些细菌。因此，我们试图建立模拟内牙龈上皮的感染器官培养模型，并旨在观察抗生素在这些建立的模型中的作用。分离小鼠背部皮肤上皮细胞，并将牙周细菌注射到上皮细胞中。将感染的上皮细胞与测试抗生素一起孵育，并评价集落形成能力。结果表明，有效的抗生素根据注射的细菌和测试的细菌组合而不同。总体而言，在器官培养模型中，阿莫西林或头孢地尼和甲硝唑的组合补偿了较低效细菌组合对彼此的影响。这项体外研究将提出有效的牙周治疗方案，特别是对于严重的牙周炎。
Mechanical reduction of infectious bacteria by using physical instruments is considered the principal therapeutic strategy for periodontal disease; addition of antibiotics is adjunctive. However, local antibiotic treatment, combined with conventional mechanical debridement, has recently been shown to be more effective in periodontitis subjects with type 2 diabetes. This suggests that some bacteria may invade the inflamed inner gingival epithelium, and mechanical debridement alone will be unable to reduce these bacteria completely. Therefore, we tried to establish infected organ culture models that mimic the inner gingival epithelium and aimed to see the effects of antibiotics in these established models. Mouse dorsal skin epithelia were isolated, and periodontal bacteria were injected into the epithelia. Infected epithelia were incubated with test antibiotics, and colony‐forming ability was evaluated. Results indicated that effective antibiotics differed according to injected bacteria and the bacterial combinations tested. Overall, in organ culture model, the combination of amoxicillin or cefdinir and metronidazole compensate for the effects of less effective bacterial combinations on each other. This in vitro study would suggest effective periodontal treatment regimens, especially for severe periodontitis.