VEGF-activated angiogenesis during bone regeneration
VEGF-activated angiogenesis during bone regeneration
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DOI：
10.1016/j.joms.2005.05.303
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发表时间：
2005-09-01
影响因子：
1.9
通讯作者：
Wiesmann, HP
中科院分区：
文献类型：
--
作者：
Kleinheinz, J;Stratmann, U;Wiesmann, HP
目的：探讨重组人血管内皮生长因子（rhVEGF（165））控释对下颌骨缺损模型血管生成和成骨的影响。材料与方法：手术56只家兔，下颌骨下缘打双皮质孔。缺损处用I型胶原蛋白、与0.8μg rhVEGF(165)复合的胶原蛋白填充，或者不进行任何填充。 3、7、14、28天后采集标本，对再生骨的血管数量、血管横截面积、面积和密度进行组织学、组织形态计量学和免疫组织学分析。结果：骨形成沿典型的向心方向发生，显示骨再生和成熟的各个阶段。新血管形成发生在成骨再生前沿之前。直到第 14 天，所有组的血管数量均有所增加，随后对照组的血管数量出现生理退化，而研究组的血管数量则持续居高不下。研究组新生骨面积与对照组无差异，但再生骨密度明显较高。结论：血管是骨形成和维持的重要组成部分，骨组织分化与局部血管的存在有关。使用 rhVEGF(165) 激活血管生成导致更强烈的血管生成和骨再生。 (c) 2005 年美国口腔颌面外科医师协会。
Purpose: The aim of this study was to investigate the influence of controlled release of recombinant human vascular endothelial growth factor (rhVEGF(165)) on angiogenesis and osteogenesis in it mandibular defect model.Material and Methods: A total of 56 rabbits were operated and bicortical holes were placed at the lower border of the mandible. The defects were filled with type-I collagen, with collagen complexed with 0.8 mu g rhVEGF(165), or left Without any filling. After 3, 7, 14, and 28 days, specimens were taken and histologic, histomorphometric, and immunohistologic analyses were carried out concerning number of vessels, cross-sectional area of vessels, and area and density, of regenerated bone.Results: Bone formation occurred in a typical centripetal direction and showed all stages of bone regeneration and maturation. New vessel formation took place in front of the osteogenic regeneration front. The number of vessels increased in all groups until day 14, followed by physiologic regression in the control groups as opposed to persisting high numbers in the study group. The area of newly formed bone showed no difference to the control group but the density of regenerated bone was significantly higher in the study group.Conclusion: Blood vessels are an important component of bone formation and maintenance and the bone tissue differentiation is related to the local presence of blood vessels. The activation of angiogenesis using rhVEGF(165) leads to more intensive angiogenesis and bone regeneration. (c) 2005 American Association of Oral and Maxillofacial Surgeons.