SYNTHETIC POLYAMINES STIMULATE IN-VITRO TRANSCRIPTION BY T7 RNA-POLYMERASE
SYNTHETIC POLYAMINES STIMULATE IN-VITRO TRANSCRIPTION BY T7 RNA-POLYMERASE
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DOI：
10.1093/nar/22.14.2784
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发表时间：
1994-07-25
影响因子：
14.9
通讯作者：
GIEGE, R
中科院分区：
文献类型：
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作者：
FRUGIER, M;FLORENTZ, C;GIEGE, R
研究了9种人工合成的多胺对T7 RNA聚合酶体外转录的影响。所用的化合物是线性或大环四胺和六胺，其大小，形状和质子化基团的数量不同。他们的效果进行了测试不同类型的模板，所有呈现T7 RNA启动子在一个双链的形式，然后由编码短转录本（25至35聚体）的序列上的单链或双链的合成寡脱氧核糖核苷酸。所有多胺用于刺激转录的两种类型的模板在水平上取决于它们的大小，形状，质子化程度和浓度。对于每种化合物，可以定义最佳浓度;高于该浓度，发生转录抑制。最大的环状化合物[38] N6 C10获得了最高的刺激（高达12倍）。
The influence of nine synthetic polyamines on in vitro transcription with T7 RNA polymerase has been studied. The compounds used were linear or macrocyclic tetra- and hexaamine, varying in their size, shape and number of protonated groups. Their effect was tested on different types of templates, all presenting the T7 RNA promoter in a double-stranded form followed by sequences encoding short transcripts (25 to 35-mers) either on single- or double-stranded synthetic oligodeoxyribonucleotides. All polyamines used stimulate transcription of both types of templates at levels dependent on their size, shape, protonation degree, and concentration. For each compound, an optimal concentration could be defined; above this concentration, transcription inhibition occurred. Highest stimulation (up to 12-fold) was obtained by the largest cyclic compound called [38]N6C10.