Amino acids in pneumolysin important for hemolytic activity identified by random mutagenesis
Amino acids in pneumolysin important for hemolytic activity identified by random mutagenesis
复制标题
通过随机诱变鉴定肺炎球菌溶血素中对溶血活性重要的氨基酸
作者：
J. Hill;P. Andrew;T. Mitchell
随机诱变溶肺素结构基因。一种用于鉴定溶血活性降低的突变体的筛选方法，在含有半胱氨酸的区域以及毒素的N端残基中发现了取代。这是首次报道的毒素n端区域内影响细胞溶解活性的变化。
The structural gene of pneumolysin was randomly mutagenized. A screen, developed to identify mutants with reduced hemolytic activity, identified substitutions within the cysteine-containing region and also at residues toward the N terminus of the toxin. These are the first reported changes within the N-terminal region of the toxin that affect cytolytic activity.