Phylogenetic and Biological Characterization of Newcastle Disease Virus Isolates from Pakistan
Phylogenetic and Biological Characterization of Newcastle Disease Virus Isolates from Pakistan
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DOI：
10.1128/jcm.00148-10
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发表时间：
2010-05-01
影响因子：
9.4
通讯作者：
Afonso, Claudio L.
中科院分区：
文献类型：
--
作者：
Khan, Taseer Ahmed;Rue, Cary A.;Afonso, Claudio L.
从巴基斯坦分离的8株纽卡斯尔病病毒进行了序列测定和特征分析。设计用于检测所有禽副粘病毒1型的PCR基质基因检测法未检测到4个分离株。一种新的基质基因检测，检测所有的菌株。系统发生分析和病理分型证实，不同基因型的强毒病毒正在巴基斯坦流行。
Eight Newcastle disease virus isolates from Pakistan were sequenced and characterized. A PCR matrix gene assay, designed to detect all avian paramyxovirus 1, did not detect four of the isolates. A new matrix gene test that detected all isolates was developed. Phylogenetic analysis and pathotyping confirmed that virulent viruses of different genotypes are circulating in Pakistan.