Dynamic compression of cartilage constructs engineered from expanded human articular chondrocytes
Dynamic compression of cartilage constructs engineered from expanded human articular chondrocytes
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DOI：
10.1016/j.bbrc.2003.09.099
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发表时间：
2003-10-17
影响因子：
3.1
通讯作者：
Martin, I
中科院分区：
文献类型：
--
作者：
Démarteau, O;Wendt, D;Martin, I
最近的研究表明，机械负荷可以改变三维支架中培养的软骨细胞的代谢活性。在这项研究中，我们确定了工程化软骨结构(扩张的成人关节软骨细胞/多活性泡沫)的发育阶段是否调节了动态压缩对糖胺多聚糖(GAG)代谢的影响。构建成熟取决于培养时间(3-14天)和供体(4个个体)。当随后施加动态加压3d时，GAG合成、累积和释放的变化与加载前构建物的GAG含量显著正相关，并导致仅在最发达的组织中刺激形成GAG。相反，这些变化与II型胶原mRNA的表达无关，表明软骨细胞对动态压缩的反应并不直接取决于细胞分化的阶段，而是依赖于细胞周围的细胞外基质。(C)2003 Elsevier Inc.保留所有权利。
Recent works have shown that mechanical loading can alter the metabolic activity of chondrocytes cultured in 3D scaffolds. In this study we determined whether the stage of development of engineered cartilaginous constructs (expanded adult human articular chondrocytes/Polyactive foams) regulates the effect of dynamic compression on glycosaminoglycan (GAG) metabolism. Construct maturation depended on the culture time (3-14 days) and the donor (4 individuals). When dynamic compression was subsequently applied for 3 days, changes in GAG synthesized, accumulated, and released were significantly positively correlated to the GAG content of the constructs prior to loading, and resulted in stimulation of GAG formation only in the most developed tissues. Conversely, none of these changes were correlated with the expression of collagen type II mRNA, indicating that the response of chondrocytes to dynamic compression does not depend directly upon the stage of cell differentiation, but rather on the extracellular matrix surrounding the cells. (C) 2003 Elsevier Inc. All rights reserved.