Expression of WT1 protein and correlation with cellular proliferation in glial tumors
Expression of WT1 protein and correlation with cellular proliferation in glial tumors
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DOI：
10.2176/nmc.47.165
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发表时间：
2007-04-01
影响因子：
1.9
通讯作者：
Yoshimine, Toshiki
中科院分区：
文献类型：
--
作者：
Hashiba, Tetsuo;Izumoto, Shuichi;Yoshimine, Toshiki
通过免疫组织化学方法研究了 73 个神经胶质瘤中 Wilms 肿瘤基因 WT1 蛋白的表达，其中包括 60 个星形细胞肿瘤、8 个少突胶质细胞肿瘤和 5 个室管膜肿瘤。在检查的 73 个神经胶质瘤中，有 70 个 (95.9%) 检测到了 WT1 蛋白。几乎所有胶质母细胞瘤、间变性星形细胞瘤、间变性室管膜瘤和间变性少突胶质细胞瘤均表达高水平的 WT1 蛋白。 WT1 蛋白表达与 MIB-1 染色指数之间存在显着相关性 (p < 0.001)。组织学检查发现WT1蛋白在间变部分以及血管周围增殖和细胞结构高的区域强烈表达，这表明WT1基因可能在胶质瘤细胞增殖中发挥重要作用。 WT1基因在各类实体瘤中过度表达，WT1蛋白是癌症免疫治疗的靶抗原。这项研究表明，许多恶性神经胶质瘤是针对 WT1 蛋白的癌症免疫治疗的良好候选者，并且 WT1 蛋白表达可用作神经胶质瘤的增殖标志物。
The expression of Wilms' tumor gene WT1 protein was investigated immunohistochemically in 73 glial tumors, including 60 astrocytic tumors, eight oligodendroglial tumors, and five ependymal tumors. WT1 protein was detected in 70 of the 73 glial tumors (95.9%) examined. Almost all glioblastomas, anaplastic astrocytomas, anaplastic ependymomas, and anaplastic oligodendrogliomas expressed high levels of WT1 protein. A significant (p < 0.001) correlation was found between WT1 protein expression and MIB-1 staining index. Histological examination found that WT1 protein was strongly expressed in the anaplastic portions and areas with perivascular proliferation and high cellularity, implying that WT1 gene might be important in glial tumor cell proliferation. WT1 gene is overexpressed in various types of solid tumors and WT1 protein is a target antigen for cancer immunotherapy. This study indicates that many malignant glial tumors are good candidates for cancer immunotherapy targeting WT1 protein and that WT1 protein expression could be used as a proliferation marker in glial tumors.